Na, K-ATPase Isoform Gene Expression in Normal and Hypertrophied Dog Heart

Overview

Journal Basic Res Cardiol

Specialty Cardiology & Vascular Diseases

Date 1996 May 1

PMID 8831945

Citations 5

Authors

R Zahler

M Gilmore-Hebert

W Sun

E J BENZ

Affiliations

Soon will be listed here.

Abstract

Objectives: The catalytic alpha subunit of the sodium-potassium ATPase, the target of digitalis glycosides, has three isoforms; the expression of these isoforms is tissue-specific and developmentally regulated. While the effect of pressure overload on Na, K-ATPase isoform expression has been studied in rodent heart, there are no systematic data on this question in hearts of larger animals, which differ from those of rodents both in isoform composition and in glycoside sensitivity. Thus, we investigated the expression of Na, K-ATPase isoforms in normal dog heart; we also examined the effect of experimental left ventricular hypertrophy on isoform expression.

Methods: hypertrophy was produced by aortic banding. Expression was assessed by quantitative Northern and Western blotting, immunofluorescence, and 3H-ouabain binding.

Results: RNA blotting indicated that the alpha 3 isoform represented 11% of Na, K-ATPase mRNA in normal dog LV. Normal dog LV expressed alpha 1 and alpha 3 protein, but no detectable alpha 2; immunoreactive alpha 1 and alpha 3 protein were also present in Purkinje fibers. There was a statistically significant decrease in total expression of all alpha isoform mRNA's in hypertrophied dog LV, resulting in a greater proportion of alpha 1. The expression level of the alpha 3 isoform mRNA and protein was lower in hypertrophied hearts.

Conclusions: These results indicate a greater proportion of alpha 1 isoform pumps in experimental canine hypertrophy. Thus, shifts in NA, K-ATPase isoforms occur in pressure-overloaded heart in large animals as well as rodents.

Citing Articles

Cardiac Oxidative Signaling and Physiological Hypertrophy in the Na/K-ATPase α1α2 Mouse Model of High Affinity for Cardiotonic Steroids.

Marck P, Pessoa M, Xu Y, Kutz L, Collins D, Yan Y Int J Mol Sci. 2021; 22(7).

PMID: 33801629 PMC: 8036649. DOI: 10.3390/ijms22073462.

The Na, K-ATPase β-Subunit Isoforms Expression in Glioblastoma Multiforme: Moonlighting Roles.

Rotoli D, Cejas M, Maeso M, Perez-Rodriguez N, Morales M, Avila J Int J Mol Sci. 2017; 18(11).

PMID: 29117147 PMC: 5713338. DOI: 10.3390/ijms18112369.

Na,K-ATPase Isozymes in Colorectal Cancer and Liver Metastases.

Bechmann M, Rotoli D, Morales M, Maeso M, Garcia M, Avila J Front Physiol. 2016; 7:9.

PMID: 26858653 PMC: 4731494. DOI: 10.3389/fphys.2016.00009.

Effects of γ-irradiation on Na,K-ATPase in cardiac sarcolemma.

Mezesova L, Vlkovicova J, Kalocayova B, Jendruchova V, Barancik M, Fulop M Mol Cell Biochem. 2013; 388(1-2):241-7.

PMID: 24347175 DOI: 10.1007/s11010-013-1915-0.

Isoform-specific stimulation of cardiac Na/K pumps by nanomolar concentrations of glycosides.

Gao J, Wymore R, Wang Y, Gaudette G, Krukenkamp I, Cohen I J Gen Physiol. 2002; 119(4):297-312.

PMID: 11929882 PMC: 2238186. DOI: 10.1085/jgp.20028501.

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