Expression of CD10/neutral Endopeptidase in Normal and Malignant Tissues of the Human Stomach and Colon

Overview

Journal J Gastroenterol

Specialty Gastroenterology

Date 1996 Feb 1

PMID 8808423

Citations 26

Authors

Y Sato

F Itoh

Y Hinoda

Y Ohe

N Nakagawa

R Ueda

A YACHI

K Imai

Affiliations

Soon will be listed here.

Abstract

The expression of common acute lymphoblastic leukemia antigen (CALLA; CD10), which is identical to neutral endopeptidase (NEP, EC3.424.11), was examined in the malignant and adjacent noninvaded tissues of the human stomach and colon (n = 27). All of 27 normal and 18 well or moderately differentiated adenocarcioma tissue specimens were positive for monoclonal antibody (mAb) NL-1 against CD10/NEP, whereas the expression level was clearly decreased in all of 9 specimens of poorly differentiated adenocarcinoma. In addition, all of 7 gastric or colorectal carcinoma cell lines tested showed decreased expression of CD10/NEP. Sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot analysis of the crude antigen J5 from the normal colon tissue lysate by mAb J5 detected a single band of approximately 100 kDa that was consistent with that of NALM-6 cells used as a positive control. These findings suggest that CD10/NEP is expressed in normal epithelial cells of the human stomach and colon, whereas the expression level is decreased in the poorly differentiated type of adenocarcinoma.

Citing Articles

CD10 Expression Correlates with Earlier Tumour Stages and Left-Sided Tumour Location in Colorectal Cancer but Has No Prognostic Impact in a European Cohort.

Grass J, Grupp K, Kluth M, Hube-Magg C, Simon R, Kemper M Cancers (Basel). 2024; 16(8).

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Predictive role of neostromal CD10 expression in breast cancer patients treated with neoadjuvant chemotherapy.

Olah O, Majlat E, Koszo R, Vereb Z, Voros A Pathol Oncol Res. 2023; 28:1610598.

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Evaluation of CD10 expression as a diagnostic marker for colorectal cancer.

Zurawski J, Talarska P, de Mezer M, Kaszkowiak K, Chalcarz M, Iwanik K Gastroenterol Hepatol Bed Bench. 2022; 15(1):24-31.

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Neutral endopeptidase depletion decreases colon cancer cell proliferation and TGF-β1 synthesis in indirect co-cultures with normal colon fibroblasts.

Mizerska-Kowalska M, Sawa-Wejksza K, Slawinska-Brych A, Kandefer-Szerszen M, Zdzisinska B Clin Transl Oncol. 2021; 23(7):1405-1414.

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Stromal CD10 expression in breast cancer correlates with tumor invasion and cancer stem cell phenotype.

Louhichi T, Saad H, Dhiab M, Ziadi S, Trimeche M BMC Cancer. 2018; 18(1):49.

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