Tandem Duplication in ErmC Translational Attenuator of the Macrolide-lincosamide-streptogramin B Resistance Plasmid PSES6 from Staphylococcus Equorum

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1996 Jan 1

PMID 8787908

Citations 8

Authors

G Lodder

S Schwarz

P Gregory

K Dyke

Affiliations

Soon will be listed here.

Abstract

A tandem duplication of 23 bp in the ermC gene translational attenuator of plasmid pSES6 from Staphylococcus equorum which mediated constitutive resistance to macrolide-lincosamide-streptogramin B antibiotics was identified. This duplication included the ribosome binding site for the ermC gene as well as the first 5 bp of the ermC coding sequence. It was postulated that this sequence duplication affects the possible RNA conformations so that the ribosome binding site for ErmC synthesis is readily accessible to the ribosomes and thus constitutive expression of the ermC gene occurs.

Citing Articles

Small Antimicrobial Resistance Plasmids in Livestock-Associated Methicillin-Resistant CC398.

Fessler A, Kadlec K, Wang Y, Zhang W, Wu C, Shen J Front Microbiol. 2018; 9:2063.

PMID: 30283407 PMC: 6157413. DOI: 10.3389/fmicb.2018.02063.

Antimicrobial Resistance among Staphylococci of Animal Origin.

Schwarz S, Fessler A, Loncaric I, Wu C, Kadlec K, Wang Y Microbiol Spectr. 2018; 6(4).

PMID: 29992898 PMC: 11633598. DOI: 10.1128/microbiolspec.ARBA-0010-2017.

Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname.

Sabat A, Hermelijn S, Akkerboom V, Juliana A, Degener J, Grundmann H Sci Rep. 2017; 7:41050.

PMID: 28106148 PMC: 5247709. DOI: 10.1038/srep41050.

The diversity of inducible and constitutively expressed erm(C) genes and association to different replicon types in staphylococci plasmids.

de Vries L, Christensen H, Agerso Y Mob Genet Elements. 2012; 2(2):72-80.

PMID: 22934240 PMC: 3429524. DOI: 10.4161/mge.20109.

Regulation of the erm(C) gene in Staphylococci from reservoir with different usage of macrolides.

Jensen L, Aarestrup F Acta Vet Scand. 2005; 46(3):163-6.

PMID: 16261929 PMC: 1624814. DOI: 10.1186/1751-0147-46-163.

References

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Weisblum B . Insights into erythromycin action from studies of its activity as inducer of resistance. Antimicrob Agents Chemother. 1995; 39(4):797-805. PMC: 162632. DOI: 10.1128/AAC.39.4.797. View

Novick R, Murphy E, Gryczan T, Baron E, Edelman I . Penicillinase plasmids of Staphylococcus aureus: restriction-deletion maps. Plasmid. 1979; 2(1):109-29. DOI: 10.1016/0147-619x(79)90010-6. View

Gryczan T, Grandi G, Hahn J, Grandi R, Dubnau D . Conformational alteration of mRNA structure and the posttranscriptional regulation of erythromycin-induced drug resistance. Nucleic Acids Res. 1980; 8(24):6081-97. PMC: 328074. DOI: 10.1093/nar/8.24.6081. View

Horinouchi S, Weisblum B . Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol. 1982; 150(2):804-14. PMC: 216433. DOI: 10.1128/jb.150.2.804-814.1982. View

Dubnau D . Translational attenuation: the regulation of bacterial resistance to the macrolide-lincosamide-streptogramin B antibiotics. CRC Crit Rev Biochem. 1984; 16(2):103-32. DOI: 10.3109/10409238409102300. View

Murphy E . Nucleotide sequence of ermA, a macrolide-lincosamide-streptogramin B determinant in Staphylococcus aureus. J Bacteriol. 1985; 162(2):633-40. PMC: 218896. DOI: 10.1128/jb.162.2.633-640.1985. View

Murphy E, Huwyler L, de Freire Bastos M . Transposon Tn554: complete nucleotide sequence and isolation of transposition-defective and antibiotic-sensitive mutants. EMBO J. 1985; 4(12):3357-65. PMC: 554666. DOI: 10.1002/j.1460-2075.1985.tb04089.x. View

Monod M, Denoya C, Dubnau D . Sequence and properties of pIM13, a macrolide-lincosamide-streptogramin B resistance plasmid from Bacillus subtilis. J Bacteriol. 1986; 167(1):138-47. PMC: 212852. DOI: 10.1128/jb.167.1.138-147.1986. View

10.

Lampson B, Parisi J . Nucleotide sequence of the constitutive macrolide-lincosamide-streptogramin B resistance plasmid pNE131 from Staphylococcus epidermidis and homologies with Staphylococcus aureus plasmids pE194 and pSN2. J Bacteriol. 1986; 167(3):888-92. PMC: 215956. DOI: 10.1128/jb.167.3.888-892.1986. View

11.

Lyon B, Skurray R . Antimicrobial resistance of Staphylococcus aureus: genetic basis. Microbiol Rev. 1987; 51(1):88-134. PMC: 373094. DOI: 10.1128/mr.51.1.88-134.1987. View

12.

Khan S, Novick R . Terminal nucleotide sequences of Tn551, a transposon specifying erythromycin resistance in Staphylococcus aureus: homology with Tn3. Plasmid. 1980; 4(2):148-54. DOI: 10.1016/0147-619x(80)90004-9. View

13.

Catchpole I, Thomas C, Davies A, DYKE K . The nucleotide sequence of Staphylococcus aureus plasmid pT48 conferring inducible macrolide-lincosamide-streptogramin B resistance and comparison with similar plasmids expressing constitutive resistance. J Gen Microbiol. 1988; 134(3):697-709. DOI: 10.1099/00221287-134-3-697. View

14.

Novick R . Staphylococcal plasmids and their replication. Annu Rev Microbiol. 1989; 43:537-65. DOI: 10.1146/annurev.mi.43.100189.002541. View

15.

Mayford M, Weisblum B . The ermC leader peptide: amino acid alterations leading to differential efficiency of induction by macrolide-lincosamide-streptogramin B antibiotics. J Bacteriol. 1990; 172(7):3772-9. PMC: 213355. DOI: 10.1128/jb.172.7.3772-3779.1990. View

16.

Catchpole I, DYKE K . A Staphylococcus aureus plasmid that specifies constitutive macrolide-lincosamide-streptogramin B resistance contains a novel deletion in the ermC attenuator. FEMS Microbiol Lett. 1990; 57(1-2):43-7. DOI: 10.1016/0378-1097(90)90410-r. View

17.

Leclercq R, Courvalin P . Bacterial resistance to macrolide, lincosamide, and streptogramin antibiotics by target modification. Antimicrob Agents Chemother. 1991; 35(7):1267-72. PMC: 245156. DOI: 10.1128/AAC.35.7.1267. View

18.

S Oliveira S, Murphy E, Gamon M, Bastos M . pRJ5: a naturally occurring Staphylococcus aureus plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a tandem duplication in the leader region of the ermC gene. J Gen Microbiol. 1993; 139(7):1461-7. DOI: 10.1099/00221287-139-7-1461. View

19.

Weisblum B, Graham M, Gryczan T, Dubnau D . Plasmid copy number control: isolation and characterization of high-copy-number mutants of plasmid pE194. J Bacteriol. 1979; 137(1):635-43. PMC: 218492. DOI: 10.1128/jb.137.1.635-643.1979. View