Structural Polypeptides of the American (VR-2332) Strain of Porcine Reproductive and Respiratory Syndrome Virus

Overview

Journal Arch Virol

Specialty Microbiology

Date 1996 Jan 1

PMID 8774694

Citations 36

Authors

E M Bautista

J J Meulenberg

C S Choi

T W Molitor

Affiliations

Soon will be listed here.

Abstract

The structural polypeptides of the isolate VR-2332 of porcine reproductive and respiratory syndrome virus were analyzed in sucrose gradient-purified virions. The virus had an average density of 1.15 g/cm3 and contained, by SDS-PAGE, three major polypeptides with apparent molecular weights of 15, 19 and 26-30 kDa, which were designated as nucleocapsid (N), matrix (M) and envelope (E), respectively. The predominant structural protein was N. N-glycosidase F digestion only affected E whereas O-glycosidase or endoglycosidase H digestion had no effect, suggesting that the viral glycoproteins contain only complex N-linked carbohydrates.

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