Structural Proteins of Equine Arteritis Virus

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1992 Nov 1

PMID 1328669

Citations 96

Authors

A A de Vries

E D Chirnside

M C Horzinek

P J Rottier

Affiliations

Soon will be listed here.

Abstract

We have recently shown that the genome of equine arteritis virus (EAV) contains seven open reading frames (ORFs). We now present data on the structural proteins of EAV and the assignment of their respective genes. Virions are composed of a 14-kDa nucleocapsid protein (N) and three membrane proteins designated M, GS, and GL. M is an unglycosylated protein of 16 kDa, and GS and GL are N-glycosylated proteins of 25 and 30 to 42 kDa, respectively. The broad size distribution of GL results from heterogeneous N-acetyllactosamine addition since it is susceptible to digestion by endo-beta-galactosidase. Using monospecific antisera as well as an antivirion serum, and by expression of individual ORFs, the genes for the structural proteins were identified: ORF 7 codes for N, ORF 6 for M, ORF 5 for GL, and ORF 2 for GS. With the exception of GS, the proteins are about equally abundant in EAV virions, being present at a molar ratio of 3 (N):2 (M):3 (GL). The GS protein, which is expressed at a level similar to that of M in infected cells, is strikingly underrepresented in virus particles (1 to 2%). Our data justify a distinct taxonomic position for EAV, together with lactate dehydrogenase-elevating virus and simian hemorrhagic fever virus; although coronavirus- and toroviruslike in features of transcription and translation, the virion architecture of EAV is fundamentally different.

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References

PORTERFIELD J, Casals J, CHUMAKOV M, GAIDAMOVICH S, Hannoun C, HOLMES I . Togaviridae. Intervirology. 1978; 9(3):129-48. DOI: 10.1159/000148930. View

Bryans J, CROWE M, DOLL E, McCollum W . Isolation of a filterable agent causing arteritis of horses and abortion by mares; its differentiation from the equine abortion (influenza) virus. Cornell Vet. 1957; 47(1):3-41. View

Trousdale M, TRENT D, Shelokov A . Simian hemorrhagic fever virus: a new togavirus. Proc Soc Exp Biol Med. 1975; 150(3):707-11. DOI: 10.3181/00379727-150-39111. View

Foxall C, Watson S, Dowbenko D, Fennie C, Lasky L, Kiso M . The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide. J Cell Biol. 1992; 117(4):895-902. PMC: 2289454. DOI: 10.1083/jcb.117.4.895. View

Kuo L, Chen Z, Rowland R, Faaberg K, Plagemann P . Lactate dehydrogenase-elevating virus (LDV): subgenomic mRNAs, mRNA leader and comparison of 3'-terminal sequences of two LDV isolates. Virus Res. 1992; 23(1-2):55-72. PMC: 7133880. DOI: 10.1016/0168-1702(92)90067-j. View

Kuo L, Harty J, Erickson L, Palmer G, Plagemann P . A nested set of eight RNAs is formed in macrophages infected with lactate dehydrogenase-elevating virus. J Virol. 1991; 65(9):5118-23. PMC: 248981. DOI: 10.1128/JVI.65.9.5118-5123.1991. View

Huntington P, Ellis P, Forman A, Timoney P . Equine viral arteritis. Aust Vet J. 1990; 67(12):429-31. DOI: 10.1111/j.1751-0813.1990.tb03050.x. View

den Boon J, Snijder E, Locker J, Horzinek M, Rottier P . Another triple-spanning envelope protein among intracellularly budding RNA viruses: the torovirus E protein. Virology. 1991; 182(2):655-63. PMC: 7130535. DOI: 10.1016/0042-6822(91)90606-c. View

de Vries A, Chirnside E, Bredenbeek P, Gravestein L, Horzinek M, Spaan W . All subgenomic mRNAs of equine arteritis virus contain a common leader sequence. Nucleic Acids Res. 1990; 18(11):3241-7. PMC: 330929. DOI: 10.1093/nar/18.11.3241. View

10.

Lee N, Wang W, Fukuda M . Granulocytic differentiation of HL-60 cells is associated with increase of poly-N-acetyllactosamine in Asn-linked oligosaccharides attached to human lysosomal membrane glycoproteins. J Biol Chem. 1990; 265(33):20476-87. View

11.

Gullick W, Downward J, Waterfield M . Antibodies to the autophosphorylation sites of the epidermal growth factor receptor protein-tyrosine kinase as probes of structure and function. EMBO J. 1985; 4(11):2869-77. PMC: 554591. DOI: 10.1002/j.1460-2075.1985.tb04016.x. View

12.

Fukuda M, Guan J, Rose J . A membrane-anchored form but not the secretory form of human chorionic gonadotropin-alpha chain acquires polylactosaminoglycan. J Biol Chem. 1988; 263(11):5314-8. View

13.

Harty J, Plagemann P . Formalin inactivation of the lactate dehydrogenase-elevating virus reveals a major neutralizing epitope not recognized during natural infection. J Virol. 1988; 62(9):3210-6. PMC: 253439. DOI: 10.1128/JVI.62.9.3210-3216.1988. View

14.

Felgner P, Gadek T, Holm M, Roman R, Chan H, Wenz M . Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure. Proc Natl Acad Sci U S A. 1987; 84(21):7413-7. PMC: 299306. DOI: 10.1073/pnas.84.21.7413. View

15.

Mumford J . Preparing for equine arteritis. Equine Vet J. 1985; 17(1):6-11. DOI: 10.1111/j.2042-3306.1985.tb02026.x. View

16.

Rottier P, Welling G, Niesters H, Lenstra J, van der Zeijst B . Predicted membrane topology of the coronavirus protein E1. Biochemistry. 1986; 25(6):1335-9. DOI: 10.1021/bi00354a022. View

17.

Cavanagh D, Davis P, Pappin D . Coronavirus IBV glycopolypeptides: locational studies using proteases and saponin, a membrane permeabilizer. Virus Res. 1986; 4(2):145-56. PMC: 7134160. DOI: 10.1016/0168-1702(86)90038-9. View

18.

Williams M, Lamb R . Polylactosaminoglycan modification of a small integral membrane glycoprotein, influenza B virus NB. Mol Cell Biol. 1988; 8(3):1186-96. PMC: 363263. DOI: 10.1128/mcb.8.3.1186-1196.1988. View

19.

KORNFELD R, Kornfeld S . Assembly of asparagine-linked oligosaccharides. Annu Rev Biochem. 1985; 54:631-64. DOI: 10.1146/annurev.bi.54.070185.003215. View

20.

Horzinek M, Maess J, Laufs R . Studies on the substructure of togaviruses. II. Analysis of equine arteritis, rubella, bovine viral diarrhea, and hog cholera viruses. Arch Gesamte Virusforsch. 1971; 33(3):306-18. View