Molecular Identification of Zeaxanthin Epoxidase of Nicotiana Plumbaginifolia, a Gene Involved in Abscisic Acid Biosynthesis and Corresponding to the ABA Locus of Arabidopsis Thaliana

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1996 May 15

PMID 8665840

Citations 173

Authors

E Marin

L Nussaume

A Quesada

M Gonneau

B Sotta

P Hugueney

A Frey

A Marion-Poll

Affiliations

Soon will be listed here.

Abstract

Abscisic acid (ABA) is a plant hormone which plays an important role in seed development and dormancy and in plant response to environmental stresses. An ABA-deficient mutant of Nicotiana plumbaginifolia, aba2, was isolated by transposon tagging using the maize Activator transposon. The aba2 mutant exhibits precocious seed germination and a severe wilty phenotype. The mutant is impaired in the first step of the ABA biosynthesis pathway, the zeaxanthin epoxidation reaction. ABA2 cDNA is able to complement N.plumbaginifolia aba2 and Arabidopsis thaliana aba mutations indicating that these mutants are homologous. ABA2 cDNA encodes a chloroplast-imported protein of 72.5 kDa, sharing similarities with different mono-oxigenases and oxidases of bacterial origin and having an ADP-binding fold and an FAD-binding domain. ABA2 protein, produced in Escherichia coli, exhibits in vitro zeaxanthin epoxidase activity. This is the first report of the isolation of a gene of the ABA biosynthetic pathway. The molecular identification of ABA2 opens the possibility to study the regulation of ABA biosynthesis and its cellular location.

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References

Hoff T, Schnorr K, Meyer C, Caboche M . Isolation of two Arabidopsis cDNAs involved in early steps of molybdenum cofactor biosynthesis by functional complementation of Escherichia coli mutants. J Biol Chem. 1995; 270(11):6100-7. DOI: 10.1074/jbc.270.11.6100. View

Weijer W, Hofsteenge J, Vereijken J, Jekel P, Beintema J . Primary structure of p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens. Biochim Biophys Acta. 1982; 704(2):385-8. DOI: 10.1016/0167-4838(82)90170-4. View

Wijnands R, Weijer W, Muller F, Jekel P, van Berkel W, Beintema J . Chemical modification of tyrosine residues in p-hydroxybenzoate hydroxylase from Pseudomonas fluorescens: assignment in sequence and catalytic involvement. Biochemistry. 1986; 25(15):4211-8. DOI: 10.1021/bi00363a007. View

Rock C, Heath T, Gage D, Zeevaart J . Abscisic alcohol is an intermediate in abscisic Acid biosynthesis in a shunt pathway from abscisic aldehyde. Plant Physiol. 1991; 97(2):670-6. PMC: 1081059. DOI: 10.1104/pp.97.2.670. View

Joshi C . Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis. Nucleic Acids Res. 1987; 15(23):9627-40. PMC: 306520. DOI: 10.1093/nar/15.23.9627. View

You I, Ghosal D, GUNSALUS I . Nucleotide sequence analysis of the Pseudomonas putida PpG7 salicylate hydroxylase gene (nahG) and its 3'-flanking region. Biochemistry. 1991; 30(6):1635-41. DOI: 10.1021/bi00220a028. View

Earp D, Lowe B, Baker B . Amplification of genomic sequences flanking transposable elements in host and heterologous plants: a tool for transposon tagging and genome characterization. Nucleic Acids Res. 1990; 18(11):3271-9. PMC: 330933. DOI: 10.1093/nar/18.11.3271. View

Giraudat J, Parcy F, Bertauche N, Gosti F, Leung J, Morris P . Current advances in abscisic acid action and signalling. Plant Mol Biol. 1994; 26(5):1557-77. DOI: 10.1007/BF00016490. View

Nurk A, Kasak L, Kivisaar M . Sequence of the gene (pheA) encoding phenol monooxygenase from Pseudomonas sp. EST1001: expression in Escherichia coli and Pseudomonas putida. Gene. 1991; 102(1):13-8. DOI: 10.1016/0378-1119(91)90531-f. View

10.

Rock C, Zeevaart J . The aba mutant of Arabidopsis thaliana is impaired in epoxy-carotenoid biosynthesis. Proc Natl Acad Sci U S A. 1991; 88(17):7496-9. PMC: 52327. DOI: 10.1073/pnas.88.17.7496. View

11.

MOORE R, Smith J . Graviresponsiveness and abscisic-acid content of roots of carotenoid-deficient mutants of Zea mays L. Planta. 1985; 164:126-8. View

12.

Kjarulff S, Okkels J . Cloning and sequencing of a full-length cDNA clone encoding the PSI-D subunit of photosystem I from barley. Plant Physiol. 1993; 101(1):335-6. PMC: 158682. DOI: 10.1104/pp.101.1.335. View

13.

von Heijne G, Steppuhn J, Herrmann R . Domain structure of mitochondrial and chloroplast targeting peptides. Eur J Biochem. 1989; 180(3):535-45. DOI: 10.1111/j.1432-1033.1989.tb14679.x. View

14.

Baker B, Coupland G, Fedoroff N, Starlinger P, Schell J . Phenotypic assay for excision of the maize controlling element Ac in tobacco. EMBO J. 1987; 6(6):1547-54. PMC: 553523. DOI: 10.1002/j.1460-2075.1987.tb02399.x. View

15.

Marion-Poll A, Marin E, Bonnefoy N, Pautot V . Transposition of the maize autonomous element Activator in transgenic Nicotiana plumbaginifolia plants. Mol Gen Genet. 1993; 238(1-2):209-17. DOI: 10.1007/BF00279549. View

16.

Rock C, Bowlby N, Hoffmann-Benning S, Zeevaart J . The aba Mutant of Arabidopsis thaliana (L.) Heynh. Has Reduced Chlorophyll Fluorescence Yields and Reduced Thylakoid Stacking. Plant Physiol. 1992; 100(4):1796-801. PMC: 1075866. DOI: 10.1104/pp.100.4.1796. View

17.

Nakahigashi K, Miyamoto K, Nishimura K, Inokuchi H . Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone. J Bacteriol. 1992; 174(22):7352-9. PMC: 207431. DOI: 10.1128/jb.174.22.7352-7359.1992. View

18.

Davies W, Tardieu F, Trejo C . How Do Chemical Signals Work in Plants that Grow in Drying Soil?. Plant Physiol. 1994; 104(2):309-314. PMC: 159200. DOI: 10.1104/pp.104.2.309. View

19.

Orser C, Lange C, Xun L, Zahrt T, SCHNEIDER B . Cloning, sequence analysis, and expression of the Flavobacterium pentachlorophenol-4-monooxygenase gene in Escherichia coli. J Bacteriol. 1993; 175(2):411-6. PMC: 196155. DOI: 10.1128/jb.175.2.411-416.1993. View

20.

Li Y, Walton D . Violaxanthin is an abscisic Acid precursor in water-stressed dark-grown bean leaves. Plant Physiol. 1990; 92(3):551-9. PMC: 1062333. DOI: 10.1104/pp.92.3.551. View