Comparative Physiology and Transcriptome Analysis Provides Insights into the Regulatory Mechanism of Albinotic

Overview

Journal Plants (Basel)

Date 2023 Dec 23

PMID 38140417

Authors

Qixia Qian

Quanfeng Ye

Yin Xu

Naresh Vasupalli

Haiwen Lu

Qiutao Hu

Dan Hou

Affiliations

Soon will be listed here.

Abstract

Albinism is a unique problem encountered in tissue culture experiments, but the underlying mechanism remains unclear in most bamboo species. In this study, we identified the putative regulatory genes in an albino mutant of using comparative physiology and transcriptome analysis. The degeneration of chloroplasts, low chlorophyll (Chl) content and reduced photosynthetic capacity were observed in albinotic compared to normal lines. A total of 6191 unigenes were identified that were clearly differentially expressed between albino and normal lines by transcriptome sequencing. Most genes related to chloroplast development (such as , ) and pigment biosynthesis (such as , , ) were downregulated significantly in albinotic lines, which might be responsible for the albino phenotype. Moreover, some transcription factors (TFs) such as PIF and GLK1 were identified to be involved in chloroplast development and Chl synthesis, indicating the involvement of putative regulatory pathways and in albinotic . Finally, the downregulation of some stress responsive TFs (like ICE1 and EREB1) suggested a reduction in stress resistance of albinotic . The above findings provided new insights into the molecular mechanism of albinism in bamboo.

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