Proflavine Sensitivity of RNA Processing in Isolated Nuclei

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1977 Mar 1

PMID 866181

Citations 8

Authors

A Yannarell

M Niemann

D E Schumm

T E Webb

Affiliations

Soon will be listed here.

Abstract

The intercalating agent proflavine inhibits the processing and subsequent release of preformed messenger RNA and ribosomal RNA from isolated liver nuclei to surrogate cytoplasm. The direct effect of proflavine on these processes, as monitored in a reconstituted cell-free system, supports the theory that base-paired segments (i.e. hairpin loops) in the precursor RNA's are involved as recognition sites in nuclear RNA processing.

Citing Articles

In vitro splicing of the ribosomal RNA precursor in isolated nucleoli from Tetrahymena.

Carin M, Jensen B, Jentsch K, Leer J, Nielsen O, Westergaard O Nucleic Acids Res. 1980; 8(23):5551-66.

PMID: 7465422 PMC: 324325. DOI: 10.1093/nar/8.23.5551.

Studies on transcription termination and splicing of the rRNA precursor in vivo in the presence of proflavine.

Nielsen O, Carin M, Westergaard O Nucleic Acids Res. 1984; 12(2):873-86.

PMID: 6694912 PMC: 318542. DOI: 10.1093/nar/12.2.873.

An assessment of some methodological criticisms of studies of RNA efflux from isolated nuclei.

Agutter P Biochem J. 1983; 214(3):915-21.

PMID: 6194787 PMC: 1152332. DOI: 10.1042/bj2140915.

Heterogeneous nuclear RNA promotes synthesis of (2',5')oligoadenylate and is cleaved by the (2',5')oligoadenylate-activated endoribonuclease.

Nilsen T, Maroney P, Robertson H, Baglioni C Mol Cell Biol. 1982; 2(2):154-60.

PMID: 6180300 PMC: 369768. DOI: 10.1128/mcb.2.2.154-160.1982.

Transcriptional arrest within the first exon is a fast control mechanism in c-myc gene expression.

Eick D, Bornkamm G Nucleic Acids Res. 1986; 14(21):8331-46.

PMID: 3537956 PMC: 311862. DOI: 10.1093/nar/14.21.8331.

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