Modified Messenger Ribonucleic Acid Release from Isolated Hepatic Nuclei After Inhibition of Polyadenylate Formation

Overview

Journal Biochem J

Specialty Biochemistry

Date 1974 Apr 1

PMID 4549426

Citations 14

Authors

D E Schumm

T E Webb

Affiliations

Soon will be listed here.

Abstract

Treatment of rats with Cordycepin (3'-deoxyadenosine), an inhibitor of nuclear poly(A) synthesis, selectively decreased the energy and cytosol-dependent release of a portion of the mRNA species from the isolated hepatic nuclei in a cell-free system by approximately 40%. An analysis of the differential binding of the transported mRNA containing poly(A) tracts to nitrocellulose filters, and to cellulose or poly(U)-Sepharose columns suggested that the poly(A) tracts in the mRNA transported from the isolated hepatic nuclei of Cordycepin-treated rats, are decreased in size. This size decrease was confirmed through an analysis of the average size of the poly(A) tracts, released from the messengers by ribonuclease activity, on sucrose density gradients.

Citing Articles

Isolation and Properties of Nuclei from Control and Auxin-treated Soybean Hypocotyl.

Chen Y, Lin C, Chang H, Guilfoyle T, Key J Plant Physiol. 1975; 56(1):78-82.

PMID: 16659261 PMC: 541301. DOI: 10.1104/pp.56.1.78.

Nuclear ribonucleoprotein release and nucleoside triphosphatase activity are inhibited by antibodies directed against one nuclear matrix glycoprotein.

Baglia F, Maul G Proc Natl Acad Sci U S A. 1983; 80(8):2285-9.

PMID: 6300906 PMC: 393804. DOI: 10.1073/pnas.80.8.2285.

Effect of cytosol on transport of RNA in vitro during cardiac hypertrophy.

Meenakshi S, Thirunavukkarasu C, Rajamanickam C Biochem J. 1983; 209(2):285-90.

PMID: 6189481 PMC: 1154092. DOI: 10.1042/bj2090285.

A comparative study of the early effects of phenobarbital and 3-methylcholanthrene on the synthesis and transport of ribonucleic acid in rat liver.

Kumar A, Rao M, Padmanaban G Biochem J. 1980; 186(1):81-7.

PMID: 6154460 PMC: 1161505. DOI: 10.1042/bj1860081.

Nutritional control of protein synthesis. Studies relating to tryptophan-induced stimulation of nucleocytoplasmic translocation of mRNA in rat liver.

SIDRANSKY H, MURTY C, VERNEY E Am J Pathol. 1984; 117(2):298-309.

PMID: 6093545 PMC: 1900445.

References

Schumm D, Webb T . Transport of informosomes from isolated nuclei of regenerating rat liver. Biochem Biophys Res Commun. 1972; 48(5):1259-65. DOI: 10.1016/0006-291x(72)90847-9. View

Rizzo A, Webb T . Regulation of ribosome formation in regenerating rat liver. Eur J Biochem. 1972; 27(1):136-44. DOI: 10.1111/j.1432-1033.1972.tb01819.x. View

Butcher F, Bushnell D, Becker J, POTTER V . Effect of cordycepin on induction of tyrosine aminotransferase employing hepatoma cells in tissue culture. Exp Cell Res. 1972; 74(1):115-23. DOI: 10.1016/0014-4827(72)90486-7. View

Yu L, Racevskis J, Webb T . Regulated transport of ribosomal subunits from regenerating rat liver nuclei in a cell-free system. Cancer Res. 1972; 32(11):2314-21. View

Adesnik M, Salditt M, Thomas W, Darnell J . Evidence that all messenger RNA molecules (except histone messenger RNA) contain Poly (A) sequences and that the Poly(A) has a nuclear function. J Mol Biol. 1972; 71(1):21-30. DOI: 10.1016/0022-2836(72)90397-x. View

Eaton B, Faulkner P . Heterogeneity in the poly(A) content of the genome of Sindbis virus. Virology. 1972; 50(3):865-73. DOI: 10.1016/0042-6822(72)90440-0. View

Lindberg U, Persson T . Isolation of mRNA from KB-cells by affinity chromatography on polyuridylic acid covalently linked to Sepharose. Eur J Biochem. 1972; 31(2):246-54. DOI: 10.1111/j.1432-1033.1972.tb02527.x. View

Sarkar P, Goldman B, Moscona A . Involvement of poly-A in selective gene expression: suppression of enzyme induction in neural retina by inhibitors of poly-A synthesis. Biochem Biophys Res Commun. 1973; 50(2):308-15. DOI: 10.1016/0006-291x(73)90841-3. View

Delarco J, Guroff G . The binding of RNA to various celluloses. Biochem Biophys Res Commun. 1973; 50(2):486-92. DOI: 10.1016/0006-291x(73)90866-8. View

10.

Sheiness D, Darnell J . Polyadenylic acid segment in mRNA becomes shorter with age. Nat New Biol. 1973; 241(113):265-8. DOI: 10.1038/newbio241265a0. View

11.

Schumm D, MORRIS H, Webb T . Cytosol-modulated transport of messenger RNA from isolated nuclei. Cancer Res. 1973; 33(8):1821-8. View

12.

Niyogi S . The influence of chain length and base composition on the specific association of oligoribonucleotides with denatured deoxyribonucleic acid. J Biol Chem. 1969; 244(6):1576-81. View

13.

Aronson A, WILT F . Properties of nuclear RNA in sea urchin embryos. Proc Natl Acad Sci U S A. 1969; 62(1):186-93. PMC: 285972. DOI: 10.1073/pnas.62.1.186. View

14.

Penman S, Rosbash M, Penman M . Messenger and heterogeneous nuclear RNA in HeLa cells: differential inhibition by cordycepin. Proc Natl Acad Sci U S A. 1970; 67(4):1878-85. PMC: 283442. DOI: 10.1073/pnas.67.4.1878. View

15.

Cooper H, Gibson E . Control of synthesis and wastage of ribosomal ribonucleic acid in lymphocytes. II. The role of protein synthesis. J Biol Chem. 1971; 246(16):5059-66. View

16.

Darnell J, Philipson L, Wall R, Adesnik M . Polyadenylic acid sequences: role in conversion of nuclear RNA into messenger RNA. Science. 1971; 174(4008):507-10. DOI: 10.1126/science.174.4008.507. View

17.

Lee S, MENDECKI J, Brawerman G . A polynucleotide segment rich in adenylic acid in the rapidly-labeled polyribosomal RNA component of mouse sarcoma 180 ascites cells. Proc Natl Acad Sci U S A. 1971; 68(6):1331-5. PMC: 389183. DOI: 10.1073/pnas.68.6.1331. View

18.

Edmonds M, VAUGHAN Jr M, Nakazato H . Polyadenylic acid sequences in the heterogeneous nuclear RNA and rapidly-labeled polyribosomal RNA of HeLa cells: possible evidence for a precursor relationship. Proc Natl Acad Sci U S A. 1971; 68(6):1336-40. PMC: 389184. DOI: 10.1073/pnas.68.6.1336. View

19.

Shearer R, SMUCKLER E . Altered regulation of the transport of RNA from nucleus to cytoplasm in rat hepatoma cells. Cancer Res. 1972; 32(2):339-42. View

20.

Brawerman G, MENDECKI J, Lee S . A procedure for the isolation of mammalian messenger ribonucleic acid. Biochemistry. 1972; 11(4):637-41. DOI: 10.1021/bi00754a027. View