Adenovirus Type 2 Coded Single-stranded DNA Binding Protein: in Vivo Phosphorylation and Modification

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1977 May 1

PMID 864833

Citations 13

Authors

Y H Jeng

W S Wold

K Sugawara

Z Gilead

M Green

Affiliations

Soon will be listed here.

Abstract

The adenovirus type 2-coded single-stranded DNA binding protein (DBP) was shown to be a phosphoprotein and to exist in at least two forms that differ in mobility by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. After a 30-min pulse with [35S]methionine or 32PO4, 35S- or 32P-labeled DBP had a nominal molecular weight of 74,000 whereas after a 30-min label followed by a 20-h chase, 35S- and 32P-labeled DBP had a nominal molecular weight of 77,000. Both large and small forms of 35S- and 32P-labeled DBP bound to single-stranded DNA-cellulose columns and were eluted by 0.4 to 0.6 M NaCl; both forms also were immunoprecipitated by antiserum against adenovirus type 1-simian virus 40-induced tumor cells (this antiserum contains antibodies against DBP) and by monospecific antiserum against 95 to 99% purified DBP. With highly purified 32P-DBP labeled 7 to 10 h postinfection, it was shown that the 32P radioactivity was firmly associated with protein material (i.e., not contaminating nucleic acids or phospholipids) and had properties expected of a phosphoester of an amino acid; paper electrophoresis of acid hydrolysates of this preparation identified phosphoserine but not phosphothreonine. Phosphoserine but not phosphothreonine was also identified in acid hydrolysates of another preparation of 32P-DBP labeled for 30 min, chased for 20 h, and then immunoprecipitated by adenovirus type 1-simian virus 40 antiserum.

Citing Articles

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Adenovirus-specific DNA-binding protein inhibits the hydrolysis of DNA by DNase in vitro.

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Characterization of the effect of aphidicolin on adenovirus DNA replication: evidence in support of a protein primer model of initiation.

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Independent mutations in Ad2ts111 cause degradation of cellular DNA and defective viral DNA replication.

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Phosphorylation of threonine in the proline-rich carboxy-terminal region of simian virus 40 large T antigen.

Scheidtmann K, Kaiser A, Carbone A, Walter G J Virol. 1981; 38(1):59-69.

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