Identification and Gene Mapping of a 14,700-molecular-weight Protein Encoded by Region E3 of Group C Adenoviruses

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1988 Jan 1

PMID 3275435

Citations 25

Authors

A E Tollefson

W S Wold

Affiliations

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Abstract

Early region E3 of adenovirus type 5 should encode at least nine proteins as judged by the DNA sequence and the spliced structures of the known mRNAs. Only two E3 proteins have been proved to exist, a glycoprotein (gp19K) and an 11,600-molecular-weight protein (11.6K protein). Here we describe an abundant 14.7K protein coded by a gene in the extreme 3' portion of E3. To identify this 14.7K protein, we constructed a bacterial vector which synthesized a TrpE-14.7K fusion protein, then we prepared antiserum against the fusion protein. This antiserum immunoprecipitated the 14.7K protein from cells infected with adenovirus types 5 and 2, as well as with a variety of E3 deletion mutants. Synthesis of the 14.7K protein correlated precisely with the presence or absence of the 14.7K gene and with the synthesis of the mRNA (mRNA h) which encodes the 14.7K protein. The 14.7K protein appeared as a triplet on immunoprecipitation gels and Western blots (immunoblots).

Citing Articles

Adenovirus E3-6.7K protein is required in conjunction with the E3-RID protein complex for the internalization and degradation of TRAIL receptor 2.

Lichtenstein D, Doronin K, Toth K, Kuppuswamy M, Wold W, Tollefson A J Virol. 2004; 78(22):12297-307.

PMID: 15507617 PMC: 525093. DOI: 10.1128/JVI.78.22.12297-12307.2004.

Distinct domains in the adenovirus E3 RIDalpha protein are required for degradation of Fas and the epidermal growth factor receptor.

Zanardi T, Yei S, Lichtenstein D, Tollefson A, Wold W J Virol. 2003; 77(21):11685-96.

PMID: 14557654 PMC: 229367. DOI: 10.1128/jvi.77.21.11685-11696.2003.

Mutations within the ADP (E3-11.6K) protein alter processing and localization of ADP and the kinetics of cell lysis of adenovirus-infected cells.

Tollefson A, Scaria A, Ying B, Wold W J Virol. 2003; 77(14):7764-78.

PMID: 12829816 PMC: 161948. DOI: 10.1128/jvi.77.14.7764-7778.2003.

Characterization of Ad5 E3-14.7K, an adenoviral inhibitor of apoptosis: structure, oligomeric state, and metal binding.

Kim H, Foster M Protein Sci. 2002; 11(5):1117-28.

PMID: 11967368 PMC: 2373546. DOI: 10.1110/ps.4180102.

Inhibition of TRAIL-induced apoptosis and forced internalization of TRAIL receptor 1 by adenovirus proteins.

Tollefson A, Toth K, Doronin K, Kuppuswamy M, Doronina O, Lichtenstein D J Virol. 2001; 75(19):8875-87.

PMID: 11533151 PMC: 114456. DOI: 10.1128/JVI.75.19.8875-8887.2001.

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