Purification and Characterization of Human Liver-specific Membrane Lipoprotein (LSP)

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1977 Mar 1

PMID 862227

Citations 75

Authors

I G McFarlane

B M Wojcicka

G M Zucker

A L Eddleston

R Williams

Affiliations

Soon will be listed here.

Abstract

A liver-specific lipoprotein (LSP) which is present in 105,000 g supernatants of human liver has previously been shown to produce a lesion resembling chronic active hepatitis in rabbits immunized with human liver fractions containing this lipoprotein. In addition, it has been implicated as the principal target antigen involved in the liver cell cytotoxicity exhibited in vitro by lymphocytes from patients with chronic active hepatitis. The organ-specificity and species cross-reactivity of LSP is confirmed. Although very labile, is has been prepared in a stable form by gel filtration of Sepharose 6B in a Tris buffer containing 1 mm disodium EDTA. LSP is also stable in a borate buffer containing EDTA but is unstable in a number of other buffer systems tested. When prepared by this method it contains approximately 2% albumin as the only detectable contaminant. Gel filtration studies on the apoprotein of LSP revealed that it has an apparent mol. wt of between 4 X 10(6) and 20 X 10(6). SDA-polyacrylamide gel electrophoresis indicated that the lipoprotein may be composed of up to thirteen sub-units of different molecular sizes.

Citing Articles

Autoantibody against a 78 kDa membrane protein of HepG2 cell in the sera of patients with alcoholic liver diseases.

Numata T, Kato J, Kohgo Y, Katsuki S, Kondo H, Niitsu Y J Gastroenterol. 1995; 30(6):751-7.

PMID: 8963393 DOI: 10.1007/BF02349642.

Changes of antigen expression on human hepatoma cell lines caused by sodium butyrate, a differentiation inducer.

Saito H, Tada S, Ebinuma H, Tsunematsu S, Kagawa T, Kumagai N J Gastroenterol. 1994; 29(6):733-9.

PMID: 7874268 DOI: 10.1007/BF02349279.

Autoantibodies against liver-specific membrane lipoprotein in acute and chronic liver diseases: studies on organ-, species-, and disease-specificity.

Manns M, Meyer zum Buschenfelde K, Hess G Gut. 1980; 21(11):955-61.

PMID: 7450561 PMC: 1419291. DOI: 10.1136/gut.21.11.955.

Immune responses to liver membrane antigens in patients with cystic fibrosis and liver disease.

Mieli-Vergani G, Psacharopoulos H, Nicholson A, Eddleston A, Mowat A, Williams R Arch Dis Child. 1980; 55(9):696-701.

PMID: 7436533 PMC: 1626989. DOI: 10.1136/adc.55.9.696.

Antibodies to a human liver membrane lipoprotein (LSP) in primary biliary cirrhosis.

Tsantoulas D, Perperas A, Portmann B, Eddleston A, Williams R Gut. 1980; 21(7):557-60.

PMID: 7429318 PMC: 1419884. DOI: 10.1136/gut.21.7.557.

References

DAMELIO V, Perlmann P . The distribution of soluble antigens in cellular structures of rat liver. Exp Cell Res. 1960; 19:383-98. DOI: 10.1016/0014-4827(60)90016-1. View

BARTLETT G . Phosphorus assay in column chromatography. J Biol Chem. 1959; 234(3):466-8. View

Perlmann P, DAMELIO V . Soluble antigens in microsomes and other cell-fractions of rat liver. Nature. 1958; 181(4607):491-2. DOI: 10.1038/181491a0. View

Poulik M . Starch gel electrophoresis in a discontinous system of buffers. Nature. 1957; 180(4600):1477-9. DOI: 10.1038/1801477a0. View

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

Miller J, Smith M, Mitchell C, REED W, Eddleston A, Williams R . Cell-mediated immunity to a human liver-specific antigen in patients with active chronic hepatitis and primary biliary cirrhosis. Lancet. 1972; 2(7772):296-7. DOI: 10.1016/s0140-6736(72)92904-2. View

Hopf U, Meyer zum Buschenfelde K, Freudenberg J . Liver-specific antigens of different species. II. Localization of a membrane antigen at cell surface of isolated hepatocytes. Clin Exp Immunol. 1974; 16(1):117-23. PMC: 1553977. View

Lenard J, Singer S . Succinylation of gamma globulin. Nature. 1966; 210(5035):536-7. DOI: 10.1038/210536a0. View

Buschenfelde K, Kossling F, MIESCHER P . Experimental chronic active hepatitis in rabbits following immunization with human liver proteins. Clin Exp Immunol. 1972; 11(1):99-108. PMC: 1553689. View

10.

Reeve C . Chemical characteristics of microsomal liver specific antigens in the rat. Br J Cancer. 1967; 21(2):401-10. PMC: 2008113. DOI: 10.1038/bjc.1967.46. View

11.

Auer I, Milgrom F . Studies on thermostable liver-specific antigens. Int Arch Allergy Appl Immunol. 1972; 42(6):871-84. DOI: 10.1159/000230664. View

12.

Cochrane A, Moussouros A, Thomsom A, Eddleston A, Wiiliams R . Antibody-dependent cell-mediated (K cell) cytotoxicity against isolated hepatocytes in chronic active hepatitis. Lancet. 1976; 1(7957):441-4. DOI: 10.1016/s0140-6736(76)91472-0. View

13.

Thomson A, Cochrane M, McFarlane I, Eddleston A, Williams R . Lymphocyte cytotoxicity to isolated hepatocytes in chronic active hepatitis. Nature. 1974; 252(5485):721-2. DOI: 10.1038/252721a0. View

14.

Helenius A, Simons K . Removal of lipids from human plasma low-density lipoprotein by detergents. Biochemistry. 1971; 10(13):2542-7. DOI: 10.1021/bi00789a019. View

15.

Fravi G, Lindenmann J . Induction by allogeneic extracts of liver-specific precipitating autoantibodies in the mouse. Nature. 1968; 218(5137):141-3. DOI: 10.1038/218141a0. View

16.

Dumonde D . Tissue-specific antigens. Adv Immunol. 1966; 5:245-412. DOI: 10.1016/s0065-2776(08)60275-8. View

17.

Smith M, Eddleston A, Williams R . Immunological factors in the evolution of active chronic hepatitis and other autoimmune liver diseases. Clin Gastroenterol. 1975; 4(2):297-313. View

18.

Hopf U, Meyer zum Buschenfelde K, Arnold W . Detection of a liver-membrane autoantibody in HBsAg-negative chronic active hepatitis. N Engl J Med. 1976; 294(11):578-82. DOI: 10.1056/NEJM197603112941103. View

19.

Hughes R, Cochrane A, Thomson A, Murray-Lyon I, Williams R . The cytotoxicity of plasma from patients with acute hepatic failure to isolated rabbit hepatocytes. Br J Exp Pathol. 1976; 57(3):348-53. PMC: 2041069. View

20.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View