Competitive ELISA for the Detection of Antibodies Against Epizootic Haemorrhagic Disease of Deer Virus

Overview

Journal J Virol Methods

Specialty Microbiology

Date 1995 Nov 1

PMID 8609206

Citations 9

Authors

J A Thevasagayam

P P Mertens

J N Burroughs

J Anderson

Affiliations

Soon will be listed here.

Abstract

A competitive enzyme-linked immunosorbent assay is described for the detection of antibodies against epizootic haemorrhagic disease of deer viruses (EHDV). Test antisera were tested against a guinea-pig antiserum raised against EHDV core particles. The assay detected antibodies to all serotypes of EHDV, but unlike the agar gel immunodiffusion (AGID) test, gave no cross-reactions with antibodies against bluetongue, Palyam and Tilligery viruses. The C-ELISA would be ideal for use in epidemiological surveys since it is suitable for the examination of antisera from all susceptible species without the need for individual species-specific enzyme conjugates.

Citing Articles

Detection of EHDV Antibodies Using Enzyme-Linked Immunosorbent Assay (ELISA).

Batten C, Newbrook K, Loundras E, Frost L Methods Mol Biol. 2024; 2838:145-153.

PMID: 39126629 DOI: 10.1007/978-1-0716-4035-7_9.

Virus Neutralization Test for Detecting and Quantifying Serum-Neutralizing Antibodies to Epizootic Hemorrhagic Disease Virus (EHDV) (Serotypes 1, 2, and 4-8).

Savini G, Bonfini B, Spedicato M Methods Mol Biol. 2024; 2838:123-136.

PMID: 39126627 DOI: 10.1007/978-1-0716-4035-7_7.

A serosurvey of bluetongue and epizootic haemorrhagic disease in a convenience sample of sheep and cattle herds in Zimbabwe.

Gordon S, Bolwell C, Rogers C, Musuka G, Kelly P, Guthrie A Onderstepoort J Vet Res. 2017; 84(1):e1-e5.

PMID: 29227131 PMC: 6238760. DOI: 10.4102/ojvr.v84i1.1505.

Development of Real-Time RT-PCR Assays for Detection and Typing of Epizootic Haemorrhagic Disease Virus.

Maan N, Maan S, Potgieter A, Wright I, Belaganahalli M, Mertens P Transbound Emerg Dis. 2016; 64(4):1120-1132.

PMID: 26888716 PMC: 5516135. DOI: 10.1111/tbed.12477.

Preparation and Characterization of a Monoclonal Antibody Against the Core Protein VP7 of the 25th Serotype of Bluetongue Virus.

Wu X, Liu Q, He J, Zang M, Wang H, Li Y Monoclon Antib Immunodiagn Immunother. 2015; 34(2):116-21.

PMID: 25897610 PMC: 4410280. DOI: 10.1089/mab.2014.0065.