Deficient Signaling in Mice Devoid of Double-stranded RNA-dependent Protein Kinase

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1995 Dec 15

PMID 8557029

Citations 277

Authors

Y L Yang

L F Reis

J Pavlovic

A Aguzzi

R Schafer

A Kumar

B R Williams

M Aguet

C Weissmann

Affiliations

Soon will be listed here.

Abstract

Double-stranded RNA-dependent protein kinase (PKR) has been implicated in interferon (IFN) induction, antiviral response and tumor suppression. We have generated mice devoid of functional PKR (Pkr%). Although the mice are physically normal and the induction of type I IFN genes by poly(I).poly(C) (pIC) and virus is unimpaired, the antiviral response induced by IFN-gamma and pIC was diminished. However, in embryo fibroblasts from Pkr knockout mice, the induction of type I IFN as well as the activation of NF-kappa B by pIC, were strongly impaired but restored by priming with IFN. Thus, PKR is not directly essential for responses to pIC, and a pIC-responsive system independent of PKR is induced by IFN. No evidence of the tumor suppressor activity of PKR was demonstrated.

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