Comparative Effects of Activation of Soluble and Particulate Guanylyl Cyclase on Cyclic GMP Elevation and Relaxation of Bovine Tracheal Smooth Muscle

Overview

Journal Br J Pharmacol

Publisher Wiley

Specialty Pharmacology

Date 1995 Jul 1

PMID 8548169

Citations 4

Authors

S C Ijioma

R A Challiss

J P Boyle

Affiliations

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Abstract

1. The effects of nitric oxide-donating compounds and atrial natriuretic peptide on cyclic GMP accumulation and mechanical tone were compared with the effects of isoprenaline in bovine tracheal smooth muscle. 2. Sodium nitroprusside, glyceryl trinitrate, S-nitroso-N-acetylpenicillamine (SNAP), atrial natriuretic peptide and isoprenaline each caused concentration-dependent inhibitions of histamine-maintained tone (EC50 values 320 +/- 80, 150 +/- 45, 14,000 +/- 4,000, 2.8 +/- 0.8 and 6.6 +/- 4.3 nM respectively). 3. When compared with their effects on histamine-induced tone, sodium nitroprusside was equally potent and effective in causing relaxation of methacholine-supported tone (EC50 290 +/- 90 nM) while isoprenaline was as effective, but less potent (EC50 30 +/- 7 nM). SNAP was more potent and equi-effective as a relaxant of methacholine-supported tone (EC50 340 +/- 140 nM). At the maximum concentrations of glyceryl trinitrate and atrial natriuretic peptide tested against methacholine-supported tone, relaxations of 52% and 14% of the isoprenaline maximum were seen. 4. Sodium nitroprusside, glyceryl trinitrate and atrial natriuretic peptide each induced concentration-dependent increases in cyclic GMP accumulation. The time-courses of accumulation correlated closely with the relaxant actions of these compounds. 5. Pretreatment of tracheal smooth muscle with sodium nitroprusside or SNAP caused a rightward shift of the concentration-effect curve for histamine while reducing the maximum response. 6. LY 83583, a putative guanylyl cyclase inhibitor, caused a concentration-dependent reduction in basal cyclic GMP accumulation in tracheal smooth muscle and inhibited the effects of sodium nitroprusside on cyclic GMP accumulation. 7. LY 83583 also inhibited the relaxation of histamine-supported tone by glyceryl trinitrate, sodium nitroprusside, SNAP and atrial natriuretic peptide, and also sodium nitroprusside- and SNAP-induced relaxation of methacholine-supported tone. However, it had no significant effect on glyceryl trinitrate-induced relaxation of methacholine-supported tone. 8. It is concluded that the relaxant actions of sodium nitroprusside, glyceryl trinitrate, SNAP and atrial natriuretic peptide follow as a result of their ability to activate either soluble or particulate guanylyl cyclase leading to cyclic GMP accumulation. Although there does not seem to be any functional difference in the relaxant response to cyclic GMP generated by the particulate as opposed to soluble form(s) of guanylyl cyclase, atrial natriuretic peptide receptor/guanylyl cyclase activation was much less effective in causing relaxation of methacholine-supported tone when compared to activators of soluble guanylyl cyclase.

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References

Hulks G, Jardine A, Connell J, Thomson N . Bronchodilator effect of atrial natriuretic peptide in asthma. BMJ. 1989; 299(6707):1081-2. PMC: 1837941. DOI: 10.1136/bmj.299.6707.1081. View

Malta E . Biphasic relaxant curves to glyceryl trinitrate in rat aortic rings. Evidence for two mechanisms of action. Naunyn Schmiedebergs Arch Pharmacol. 1989; 339(1-2):236-43. DOI: 10.1007/BF00165149. View

Hulks G, Jardine A, Connell J, Thomson N . Effect of atrial natriuretic factor on bronchomotor tone in the normal human airway. Clin Sci (Lond). 1990; 79(1):51-5. DOI: 10.1042/cs0790051. View

Fedan J, NUTT M, Frazer D . Reactivity of guinea-pig isolated trachea to methacholine, histamine and isoproterenol applied serosally versus mucosally. Eur J Pharmacol. 1990; 190(3):337-45. DOI: 10.1016/0014-2999(90)94198-7. View

FURCHGOTT R, Jothianandan D . Endothelium-dependent and -independent vasodilation involving cyclic GMP: relaxation induced by nitric oxide, carbon monoxide and light. Blood Vessels. 1991; 28(1-3):52-61. DOI: 10.1159/000158843. View

Li C, Rand M . Evidence that part of the NANC relaxant response of guinea-pig trachea to electrical field stimulation is mediated by nitric oxide. Br J Pharmacol. 1991; 102(1):91-4. PMC: 1917893. DOI: 10.1111/j.1476-5381.1991.tb12137.x. View

Chilvers E, Giembycz M, Challiss R, Barnes B, Nahorski S . Lack of effect of zaprinast on methacholine-induced contraction and inositol 1,4,5-trisphosphate accumulation in bovine tracheal smooth muscle. Br J Pharmacol. 1991; 103(1):1119-25. PMC: 1908075. DOI: 10.1111/j.1476-5381.1991.tb12310.x. View

Chinkers M, Garbers D . Signal transduction by guanylyl cyclases. Annu Rev Biochem. 1991; 60:553-75. DOI: 10.1146/annurev.bi.60.070191.003005. View

Zhou H, Torphy T . Relationship between cyclic guanosine monophosphate accumulation and relaxation of canine trachealis induced by nitrovasodilators. J Pharmacol Exp Ther. 1991; 258(3):972-8. View

10.

Giembycz M, Raeburn D . Putative substrates for cyclic nucleotide-dependent protein kinases and the control of airway smooth muscle tone. J Auton Pharmacol. 1991; 11(6):365-98. DOI: 10.1111/j.1474-8673.1991.tb00260.x. View

11.

Kannan M, Johnson D . Nitric oxide mediates the neural nonadrenergic, noncholinergic relaxation of pig tracheal smooth muscle. Am J Physiol. 1992; 262(4 Pt 1):L511-4. DOI: 10.1152/ajplung.1992.262.4.L511. View

12.

Jansen A, Drazen J, Osborne J, Brown R, Loscalzo J, Stamler J . The relaxant properties in guinea pig airways of S-nitrosothiols. J Pharmacol Exp Ther. 1992; 261(1):154-60. View

13.

Bates J, Baker M, Guerra Jr R, Harrison D . Nitric oxide generation from nitroprusside by vascular tissue. Evidence that reduction of the nitroprusside anion and cyanide loss are required. Biochem Pharmacol. 1991; 42 Suppl:S157-65. DOI: 10.1016/0006-2952(91)90406-u. View

14.

Belvisi M, Stretton C, Yacoub M, Barnes P . Nitric oxide is the endogenous neurotransmitter of bronchodilator nerves in humans. Eur J Pharmacol. 1992; 210(2):221-2. DOI: 10.1016/0014-2999(92)90676-u. View

15.

Kowaluk E, Seth P, Fung H . Metabolic activation of sodium nitroprusside to nitric oxide in vascular smooth muscle. J Pharmacol Exp Ther. 1992; 262(3):916-22. View

16.

Tobin A, Lambert D, Nahorski S . Rapid desensitization of muscarinic m3 receptor-stimulated polyphosphoinositide responses. Mol Pharmacol. 1992; 42(6):1042-8. View

17.

Angus R, McCallum M, Hulks G, Thomson N . Bronchodilator, cardiovascular, and cyclic guanylyl monophosphate response to high-dose infused atrial natriuretic peptide in asthma. Am Rev Respir Dis. 1993; 147(5):1122-5. DOI: 10.1164/ajrccm/147.5.1122. View

18.

Harrison D, Bates J . The nitrovasodilators. New ideas about old drugs. Circulation. 1993; 87(5):1461-7. DOI: 10.1161/01.cir.87.5.1461. View

19.

Hsu K, Fu W, Lin-Shiau S . Blockade by 2,2',2''-tripyridine of the nicotinic acetylcholine receptor channels in embryonic Xenopus muscle cells. Br J Pharmacol. 1993; 110(1):163-8. PMC: 2176001. DOI: 10.1111/j.1476-5381.1993.tb13787.x. View

20.

Seth P, Fung H . Biochemical characterization of a membrane-bound enzyme responsible for generating nitric oxide from nitroglycerin in vascular smooth muscle cells. Biochem Pharmacol. 1993; 46(8):1481-6. DOI: 10.1016/0006-2952(93)90115-d. View