Regulation of Adenosine Receptors in Cultured Heart Cells

Overview

Journal Adv Exp Med Biol

Specialties Biology
General Medicine

Date 1995 Jan 1

PMID 8540397

Citations 3

Authors

D El-Ani

K A Jacobson

A Shainberg

Affiliations

Soon will be listed here.

Abstract

A1 adenosine receptors were studied on heart cells grown in cultures by the radioligand binding technique. Treatments with agents that accelerated heart rate for 3-4 days, caused an increase in the level of adenosine receptors. Treatments that attenuated heart rate, reduced the level of the receptors. Thus, the cardiac cells respond to environmental conditions affecting heart contraction so as to restore the basal rate of heart activity.

Citing Articles

Uptake of glucose analogs reflects the rate of contraction of cultured myocytes.

El-Ani D, Jacobson K, Zimlichman R, Katz A, Shainberg A J Basic Clin Physiol Pharmacol. 2000; 10(4):287-303.

PMID: 10631593 PMC: 10794912. DOI: 10.1515/jbcpp.1999.10.4.287.

Regulation of A1 adenosine receptors by amiodarone and electrical stimulation in rat myocardial cells in vitro.

El-Ani D, Jacobson K, Shainberg A Biochem Pharmacol. 1997; 54(5):583-7.

PMID: 9337074 PMC: 5470623. DOI: 10.1016/s0006-2952(97)00177-9.

Effects of theophylline and dibutyryl-cAMP on adenosine receptors and heart rate in cultured cardiocytes.

El-Ani D, Jacobson K, Shainberg A J Basic Clin Physiol Pharmacol. 1996; 7(4):347-62.

PMID: 9080309 PMC: 5472055. DOI: 10.1515/jbcpp.1996.7.4.347.

References

Zhou Q, Li C, Olah M, Johnson R, Stiles G, Civelli O . Molecular cloning and characterization of an adenosine receptor: the A3 adenosine receptor. Proc Natl Acad Sci U S A. 1992; 89(16):7432-6. PMC: 49724. DOI: 10.1073/pnas.89.16.7432. View

van Calker D, Muller M, Hamprecht B . Adenosine regulates via two different types of receptors, the accumulation of cyclic AMP in cultured brain cells. J Neurochem. 1979; 33(5):999-1005. DOI: 10.1111/j.1471-4159.1979.tb05236.x. View

Dobson Jr J . Mechanism of adenosine inhibition of catecholamine-induced responses in heart. Circ Res. 1983; 52(2):151-60. DOI: 10.1161/01.res.52.2.151. View

Belardinelli L, Isenberg G . Actions of adenosine and isoproterenol on isolated mammalian ventricular myocytes. Circ Res. 1983; 53(3):287-97. DOI: 10.1161/01.res.53.3.287. View

Brik H, Shainberg A . Thyroxine induces transition of red towards white muscle in cultured heart cells. Basic Res Cardiol. 1990; 85(3):237-46. DOI: 10.1007/BF01907112. View

Kukovetz W, Poch G, Wurm A . Quantitative relations between cyclic AMP and contraction as affected by stimulators of adenylate cyclase and inhibitors of phosphodiesterase. Adv Cyclic Nucleotide Res. 1975; 5:395-414. View

Wu S, Linden J, Visentin S, BOYKIN M, Belardinelli L . Enhanced sensitivity of heart cells to adenosine and up-regulation of receptor number after treatment of guinea pigs with theophylline. Circ Res. 1989; 65(4):1066-77. DOI: 10.1161/01.res.65.4.1066. View

Leung E, Jacobson K, Green R . Apparent heterogeneity of cardiac A1 adenosine receptors as revealed by radioligand binding experiments on N-ethylmaleimide-treated membranes. Naunyn Schmiedebergs Arch Pharmacol. 1991; 344(6):639-44. PMC: 5870127. DOI: 10.1007/BF00174747. View

Liang B, Hirsch A . Homologous sensitisation of embryonic chick atrial myocytes to adenosine: mediation by adenosine A1 receptor and guanine nucleotide binding protein. Cardiovasc Res. 1993; 27(1):102-10. DOI: 10.1093/cvr/27.1.102. View

10.

Liang B . Characterization of the adenosine receptor in cultured embryonic chick atrial myocytes: coupling to modulation of contractility and adenylate cyclase activity and identification by direct radioligand binding. J Pharmacol Exp Ther. 1989; 249(3):775-84. View

11.

Shainberg A, Burstein M . Decrease of acetylcholine receptor synthesis in muscle cultures by electrical stimulation. Nature. 1976; 264(5584):368-9. DOI: 10.1038/264368a0. View

12.

Shryock J, Patel A, Belardinelli L, Linden J . Downregulation and desensitization of A1-adenosine receptors in embryonic chicken heart. Am J Physiol. 1989; 256(2 Pt 2):H321-7. DOI: 10.1152/ajpheart.1989.256.2.H321. View

13.

Kurachi Y, Nakajima T, Sugimoto T . On the mechanism of activation of muscarinic K+ channels by adenosine in isolated atrial cells: involvement of GTP-binding proteins. Pflugers Arch. 1986; 407(3):264-74. DOI: 10.1007/BF00585301. View

14.

El-Ani D, Jacobson K, Shainberg A . Characterization of adenosine receptors in intact cultured heart cells. Biochem Pharmacol. 1994; 48(4):727-35. PMC: 5610786. DOI: 10.1016/0006-2952(94)90050-7. View

15.

Linden J, Hollen C, Patel A . The mechanism by which adenosine and cholinergic agents reduce contractility in rat myocardium. Correlation with cyclic adenosine monophosphate and receptor densities. Circ Res. 1985; 56(5):728-35. DOI: 10.1161/01.res.56.5.728. View

16.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

17.

Fredholm B . On the mechanism of action of theophylline and caffeine. Acta Med Scand. 1985; 217(2):149-53. DOI: 10.1111/j.0954-6820.1985.tb01650.x. View

18.

Collis M . Evidence for an A1-adenosine receptor in the guinea-pig atrium. Br J Pharmacol. 1983; 78(1):207-12. PMC: 2044793. DOI: 10.1111/j.1476-5381.1983.tb09381.x. View

19.

Longabaugh J, Didsbury J, Spiegel A, Stiles G . Modification of the rat adipocyte A1 adenosine receptor-adenylate cyclase system during chronic exposure to an A1 adenosine receptor agonist: alterations in the quantity of GS alpha and Gi alpha are not associated with changes in their mRNAs. Mol Pharmacol. 1989; 36(5):681-8. View

20.

Londos C, Cooper D, Wolff J . Subclasses of external adenosine receptors. Proc Natl Acad Sci U S A. 1980; 77(5):2551-4. PMC: 349439. DOI: 10.1073/pnas.77.5.2551. View