Carbon Catabolite Regulation of Transcription of Nuclear Genes Coding for Mitochondrial Proteins in the Yeast Kluyveromyces Lactis

Overview

Journal Curr Genet

Specialty Genetics

Date 1995 Aug 1

PMID 8529273

Citations 8

Authors

W Mulder

I H Scholten

L A Grivell

Affiliations

Soon will be listed here.

Abstract

Promoter regions of the KlQCR7, KlQCR8 and KlCYC1 genes, coding for subunits of the bc1-complex and cytochrome c respectively, in the short-term Crabtree-negative yeast Kluyveromyces lactis differ markedly in sequence from their Saccharomyces cerevisiae counterparts. They have, however, conserved very similar configurations of binding-site motifs for various transcription factors known to be involved in global and carbon-source regulation in S. cerevisiae. To investigate the carbon source-dependent expression of these genes in K. lactis, we have carried out medium-shift experiments and determined transcript levels during the shifts. In sharp contrast to the situation in S. cerevisiae, the level of expression in K. lactis is not affected when glucose is added to a non-fermentable carbon-source medium. However, the genes are not constitutively expressed, but become significantly induced when the cells are shifted from glucose to a non-fermentable carbon source. Finally, induction of transcriptional activation does not occur in media containing both glucose and non-fermentable carbon sources.

Citing Articles

Proteomic and functional consequences of hexokinase deficiency in glucose-repressible Kluyveromyces lactis.

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Identifying cis-regulatory changes involved in the evolution of aerobic fermentation in yeasts.

Lin Z, Wang T, Tsai B, Wu F, Yu F, Tseng Y Genome Biol Evol. 2013; 5(6):1065-78.

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Protective vaccination against infectious bursal disease virus with whole recombinant Kluyveromyces lactis yeast expressing the viral VP2 subunit.

Arnold M, Durairaj V, Mundt E, Schulze K, Breunig K, Behrens S PLoS One. 2012; 7(9):e42870.

PMID: 23024743 PMC: 3443089. DOI: 10.1371/journal.pone.0042870.

A novel, lactase-based selection and strain improvement strategy for recombinant protein expression in Kluyveromyces lactis.

Krijger J, Baumann J, Wagner M, Schulze K, Reinsch C, Klose T Microb Cell Fact. 2012; 11:112.

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Regulation of phosphatidylglycerolphosphate synthase in aerobic yeast Kluyveromyces lactis.

Ticha E, Polakovicova V, Obernauerova M Folia Microbiol (Praha). 2008; 53(4):319-24.

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