Control of MRNA Turnover As a Mechanism of Glucose Repression in Saccharomyces Cerevisiae

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1992 Jul 1

PMID 1620107

Citations 22

Authors

A Lombardo

G P Cereghino

I E Scheffler

Affiliations

Soon will be listed here.

Abstract

We have examined the expression of the gene encoding the iron-protein subunit (Ip) of succinate dehydrogenase in Saccharomyces cerevisiae. The gene had been cloned by us and shown to be subject to glucose regulation (A. Lombardo, K. Carine, and I. E. Scheffler, J. Biol. Chem. 265:10419-10423, 1990). We discovered that a significant part of the regulation of the Ip mRNA levels by glucose involves the regulation of the turnover rate of this mRNA. In the presence of glucose, the half-life appears to be less than 5 min, while in glycerol medium, the half-life is greater than 60 min. The gene is also regulated transcriptionally by glucose. The upstream promoter sequence appeared to have four regulatory elements with consensus sequences shown to be responsible for the interaction with the HAP2/3/4 regulatory complex. A deletion analysis has shown that the two distal elements are redundant. These measurements were carried out by Northern (RNA) analyses of Ip mRNA transcripts as well as by assays of beta-galactosidase activity in cells carrying constructs of the Ip promoter linked to the lacZ coding sequence. These observations on the regulation of mRNA stability were also extended to the mRNA of the flavoprotein subunit of succinate dehydrogenase and in some experiments of iso-1-cytochrome c.

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References

Simoes E, Sarnow P . An RNA hairpin at the extreme 5' end of the poliovirus RNA genome modulates viral translation in human cells. J Virol. 1991; 65(2):913-21. PMC: 239832. DOI: 10.1128/JVI.65.2.913-921.1991. View

Wright R, Poyton R . Release of two Saccharomyces cerevisiae cytochrome genes, COX6 and CYC1, from glucose repression requires the SNF1 and SSN6 gene products. Mol Cell Biol. 1990; 10(3):1297-300. PMC: 361024. DOI: 10.1128/mcb.10.3.1297-1300.1990. View

Williams F, Trumbly R . Characterization of TUP1, a mediator of glucose repression in Saccharomyces cerevisiae. Mol Cell Biol. 1990; 10(12):6500-11. PMC: 362927. DOI: 10.1128/mcb.10.12.6500-6511.1990. View

Chodosh L, Olesen J, Hahn S, Baldwin A, Guarente L, Sharp P . A yeast and a human CCAAT-binding protein have heterologous subunits that are functionally interchangeable. Cell. 1988; 53(1):25-35. DOI: 10.1016/0092-8674(88)90484-9. View

HATEFI Y . The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem. 1985; 54:1015-69. DOI: 10.1146/annurev.bi.54.070185.005055. View

Emr S, Vassarotti A, Garrett J, Geller B, Takeda M, DOUGLAS M . The amino terminus of the yeast F1-ATPase beta-subunit precursor functions as a mitochondrial import signal. J Cell Biol. 1986; 102(2):523-33. PMC: 2114072. DOI: 10.1083/jcb.102.2.523. View

Hahn S, Guarente L . Yeast HAP2 and HAP3: transcriptional activators in a heteromeric complex. Science. 1988; 240(4850):317-21. DOI: 10.1126/science.2832951. View

Maarse A, de Haan M, Bout A, Grivell L . Demarcation of a sequence involved in mediating catabolite repression of the gene for the 11 kDa subunit VIII of ubiquinol-cytochrome c oxidoreductase in Saccharomyces cerevisiae. Nucleic Acids Res. 1988; 16(13):5797-811. PMC: 336830. DOI: 10.1093/nar/16.13.5797. View

Olesen J, Hahn S, Guarente L . Yeast HAP2 and HAP3 activators both bind to the CYC1 upstream activation site, UAS2, in an interdependent manner. Cell. 1987; 51(6):953-61. DOI: 10.1016/0092-8674(87)90582-4. View

10.

Sakai A, Shimizu Y, Kondou S, Chibazakura T, Hishinuma F . Structure and molecular analysis of RGR1, a gene required for glucose repression of Saccharomyces cerevisiae. Mol Cell Biol. 1990; 10(8):4130-8. PMC: 360937. DOI: 10.1128/mcb.10.8.4130-4138.1990. View

11.

Lombardo A, Carine K, Scheffler I . Cloning and characterization of the iron-sulfur subunit gene of succinate dehydrogenase from Saccharomyces cerevisiae. J Biol Chem. 1990; 265(18):10419-23. View

12.

Herrick D, Parker R, Jacobson A . Identification and comparison of stable and unstable mRNAs in Saccharomyces cerevisiae. Mol Cell Biol. 1990; 10(5):2269-84. PMC: 360574. DOI: 10.1128/mcb.10.5.2269-2284.1990. View

13.

van Loon A, van Eijk E, Grivell L . Biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. Discoordinate synthesis of the 11-kd subunit in response to increased gene copy number. EMBO J. 1983; 2(10):1765-70. PMC: 555356. DOI: 10.1002/j.1460-2075.1983.tb01655.x. View

14.

Guarente L, Mason T . Heme regulates transcription of the CYC1 gene of S. cerevisiae via an upstream activation site. Cell. 1983; 32(4):1279-86. DOI: 10.1016/0092-8674(83)90309-4. View

15.

Guarente L, Ptashne M . Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A. 1981; 78(4):2199-203. PMC: 319311. DOI: 10.1073/pnas.78.4.2199. View

16.

Ito H, Fukuda Y, Murata K, Kimura A . Transformation of intact yeast cells treated with alkali cations. J Bacteriol. 1983; 153(1):163-8. PMC: 217353. DOI: 10.1128/jb.153.1.163-168.1983. View

17.

Zitomer R, Montgomery D, Nichols D, Hall B . Transcriptional regulation of the yeast cytochrome c gene. Proc Natl Acad Sci U S A. 1979; 76(8):3627-31. PMC: 383885. DOI: 10.1073/pnas.76.8.3627. View

18.

Zitomer R, Nichols D . Kinetics of glucose repression of yeast cytochrome c. J Bacteriol. 1978; 135(1):39-44. PMC: 224761. DOI: 10.1128/jb.135.1.39-44.1978. View

19.

SINGER T, Johnson M . The prosthetic groups of succinate dehydrogenase: 30 years from discovery to identification. FEBS Lett. 1985; 190(2):189-98. DOI: 10.1016/0014-5793(85)81282-5. View

20.

Forsburg S, Guarente L . Communication between mitochondria and the nucleus in regulation of cytochrome genes in the yeast Saccharomyces cerevisiae. Annu Rev Cell Biol. 1989; 5:153-80. DOI: 10.1146/annurev.cb.05.110189.001101. View