Purification and Preliminary X-ray Crystallographic Studies of Recombinant L-ribulose-5-phosphate 4-epimerase from Escherichia Coli

Overview

Journal Protein Sci

Specialty Biochemistry

Date 1995 Aug 1

PMID 8520491

Citations 2

Authors

A Andersson

G Schneider

Y Lindqvist

Affiliations

Soon will be listed here.

Abstract

The araD gene from Escherichia coli, coding for L-ribulose-5-phosphate 4-epimerase, was overexpressed and the resulting enzyme was purified to homogeneity. Crystals of L-ribulose-5-phosphate 4-epimerase, obtained with 4.0 M sodium formate as precipitant, belong to space group P4212 with unit cell dimensions a = b = 107.8 A and c = 281.4 A and diffract to at least 2.2 A resolution. Density measurements of these crystals are consistent with eight subunits in the asymmetric unit.

Citing Articles

Identification and analysis of the paulomycin biosynthetic gene cluster and titer improvement of the paulomycins in Streptomyces paulus NRRL 8115.

Li J, Xie Z, Wang M, Ai G, Chen Y PLoS One. 2015; 10(3):e0120542.

PMID: 25822496 PMC: 4425429. DOI: 10.1371/journal.pone.0120542.

Utilization of D-ribitol by Lactobacillus casei BL23 requires a mannose-type phosphotransferase system and three catabolic enzymes.

Bourand A, Yebra M, Boel G, Maze A, Deutscher J J Bacteriol. 2013; 195(11):2652-61.

PMID: 23564164 PMC: 3676056. DOI: 10.1128/JB.02276-12.

References

Lee N, Patrick J, Masson M . Crystalline L-ribulose 5-phosphate 4-epimerase from Escherichia coli. J Biol Chem. 1968; 243(18):4700-5. View

Matthews B . Solvent content of protein crystals. J Mol Biol. 1968; 33(2):491-7. DOI: 10.1016/0022-2836(68)90205-2. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

BURMA D, HORECKER B . Pentose fermenation by Lactobacillus plantarum. IV. L-ribulose-5-phosphate 4-epimerase. J Biol Chem. 1958; 231(2):1053-64. View

Bode W, Schirmer T . Determination of the protein content of crystals formed by Mastigocladus laminosus C-phycocyanin, Chroomonas spec. phycocyanin-645 and modified human fibrinogen using an improved Ficoll density gradient method. Biol Chem Hoppe Seyler. 1985; 366(3):287-95. DOI: 10.1515/bchm3.1985.366.1.287. View