Purification and Properties of D-ribulose-5-phosphate 3-epimerase from Calf Liver

Overview

Journal Biochim Biophys Acta

Specialties Biochemistry
Biophysics

Date 1979 Oct 11

PMID 497230

Citations 12

Authors

T Wood

Affiliations

Soon will be listed here.

Abstract

D-Ribulose-5-phosphate 3-epimerase (EC 5.1.3.1) was purified 760-fold from calf liver by adsorption on DEAE-cellulose, chromatography on DEAE-Sephadex, chromatography on D-ribose 5-phosphate-Sepharose and gel filtration on Biogel P200. The purified enzyme of specific activity 617 units/mg was obtained in 28% yield and gave a single band on polyacrylamide gel electrophoresis. It had a molecular weight of 45 000 and appeared to contain two identical peptide chains of 22 900 daltons. The Km for D-ribulose 5-phosphate was 0.19 +/- 0.07 mM (S.E.). It was inhibited by reagents reacting with sulphydryl groups, by sulphate ion, and by D-deoxyribose 5-phosphate. The pH-stability and pH-activity curves were determined.

Citing Articles

CSGID Solves Structures and Identifies Phenotypes for Five Enzymes in .

Lykins J, Filippova E, Halavaty A, Minasov G, Zhou Y, Dubrovska I Front Cell Infect Microbiol. 2018; 8:352.

PMID: 30345257 PMC: 6182094. DOI: 10.3389/fcimb.2018.00352.

Structure, kinetic characterization and subcellular localization of the two ribulose 5-phosphate epimerase isoenzymes from Trypanosoma cruzi.

Gonzalez S, Valsecchi W, Maugeri D, Delfino J, Cazzulo J PLoS One. 2017; 12(2):e0172405.

PMID: 28207833 PMC: 5312968. DOI: 10.1371/journal.pone.0172405.

Molecular biology of the C3 photosynthetic carbon reduction cycle.

Raines C, Lloyd J, Dyer T Photosynth Res. 2014; 27(1):1-14.

PMID: 24414440 DOI: 10.1007/BF00029971.

Analysis of Chinese hamster ovary cell metabolism through a combined computational and experimental approach.

Chen N, Bennett M, Kontoravdi C Cytotechnology. 2013; 66(6):945-66.

PMID: 24292563 PMC: 4235950. DOI: 10.1007/s10616-013-9648-1.

Molar absorptivity and A1 cm (1%) values for proteins at selected wavelengths of the ultraviolet and visible regions. XXII.

Kirschenbaum D Appl Biochem Biotechnol. 2013; 7(6):475-95.

PMID: 24234461 DOI: 10.1007/BF02799179.