Mutational Analysis of the Leucine Zipper-like Motif of the Human Immunodeficiency Virus Type 1 Envelope Transmembrane Glycoprotein

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1993 Jun 1

PMID 8497069

Citations 51

Authors

S S Chen

C N Lee

W R Lee

K McIntosh

T H Lee

Affiliations

Soon will be listed here.

Abstract

The N-terminal region of the envelope (env) transmembrane protein of human immunodeficiency virus type 1 (HIV-1) has a leucine zipper-like motif. This highly conserved zipper motif, which consists of a heptad repeat of leucine or isoleucine residues, has been suggested to play a role in HIV-1 env glycoprotein oligomerization. This hypothesis was tested by replacing the highly conserved leucine or isoleucine residues in the zipper motif with a strong alpha-helix breaker, proline. We report here that such substitutions did not abolish the ability of env protein to form oligomers, indicating that this highly conserved zipper motif does not have a crucial role in env protein oligomerization. However, the mutant viruses all showed impaired infectivity, suggesting that this conserved zipper motif can have an important role in the virus life cycle.

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