Microdilution Transfer Plate Technique for Determining in Vitro Synergy of Antimicrobial Agents

Overview

Journal Antimicrob Agents Chemother

Publisher American Society for Microbiology

Specialty Pharmacology

Date 1977 Feb 1

PMID 848924

Citations 15

Authors

P F Dougherty

D W Yotter

T R Matthews

Affiliations

Soon will be listed here.

Abstract

A microdilution transfer plate technique for determining in vitro synergy of antimicrobial agents is described. Combinations of gentamicin-nalidixic acid against Proteus mirabilis and rifampin-amphotericin B against Candida albicans are used as examples to demonstrate the technique. Results correlate with published data obtained by conventional methods. The technique is effective for evaluating the in vitro effects of antimicrobial agent combinations against both bacteria and fungi. The technique enables one to produce a checkerboard gradient in a fast, convenient, and reproducible way; results are easily visualized.

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