Sequence Divergence in Two Tandemly Located Pilin Genes of Eikenella Corrodens

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1993 May 1

PMID 8478080

Citations 6

Authors

T Tonjum

S Weir

K BOVRE

A Progulske-Fox

C F Marrs

Affiliations

Soon will be listed here.

Abstract

Eikenella corrodens normally inhabits the human respiratory and gastrointestinal tracts but is frequently the cause of abscesses at various sites. Using the N-terminal portion of the Moraxella nonliquefaciens pilin gene as a hybridization probe, we cloned two tandemly located pilin genes of E. corrodens 31745, ecpC and ecpD, and expressed the two pilin genes separately in Escherichia coli. A comparison of the predicted amino acid sequences of E. corrodens 31745 EcpC and EcpD revealed considerable divergence between the sequences of these two pilins and even less similarity to EcpA and EcpB of E. corrodens type strain ATCC 23834. EcpC from E. corrodens 31745 displayed high degrees of homology to the pilins of Neisseria gonorrhoeae and Pseudomonas aeruginosa. EcpD from E. corrodens 31745 showed the highest homologies with the pilin of one of the three P. aeruginosa classes, whereas EcpA and EcpB of strain ATCC 23834 most closely resemble Moraxella bovis pilins. These findings raise interesting questions about potential genetic transfer between different bacterial species, as opposed to convergent evolution.

Citing Articles

Neisseria cinerea isolates can adhere to human epithelial cells by type IV pilus-independent mechanisms.

Wormann M, Horien C, Johnson E, Liu G, Aho E, Tang C Microbiology (Reading). 2016; 162(3):487-502.

PMID: 26813911 PMC: 4891991. DOI: 10.1099/mic.0.000248.

Cloning, purification, crystallization and preliminary X-ray diffraction studies of Escherichia coli PapD-like protein (EcpD).

Pandey N, Pal R, Kashyap M, Bhavesh N Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012; 68(Pt 8):954-7.

PMID: 22869131 PMC: 3412782. DOI: 10.1107/S1744309112027364.

Identification of neisserial DNA binding components.

Lang E, Haugen K, Fleckenstein B, Homberset H, Frye S, Ambur O Microbiology (Reading). 2009; 155(Pt 3):852-862.

PMID: 19246756 PMC: 2885667. DOI: 10.1099/mic.0.022640-0.

Eikenella corrodens phase variation involves a posttranslational event in pilus formation.

Villar M, Helber J, Hood B, Schaefer M, Hirschberg R J Bacteriol. 1999; 181(14):4154-60.

PMID: 10400570 PMC: 93914. DOI: 10.1128/JB.181.14.4154-4160.1999.

Identification of four complete type 4 pilin genes in a single Kingella denitrificans genome.

Weir S, Lee L, Marrs C Infect Immun. 1996; 64(12):4993-9.

PMID: 8945537 PMC: 174479. DOI: 10.1128/iai.64.12.4993-4999.1996.

References

HENRIKSEN S . Corroding bacteria from the respiratory tract. 2. Bacteroides corrodens. Acta Pathol Microbiol Scand. 1969; 75(1):91-6. View

Marrs C, Schoolnik G, Koomey J, Hardy J, Rothbard J, Falkow S . Cloning and sequencing of a Moraxella bovis pilin gene. J Bacteriol. 1985; 163(1):132-9. PMC: 219090. DOI: 10.1128/jb.163.1.132-139.1985. View

Brooks G, ODonoghue J, Rissing J, Soapes K, Smith J . Eikenella corrodens, a recently recognized pathogen: infections in medical-surgical patients and in association with methylphenidate abuse. Medicine (Baltimore). 1974; 53(5):325-42. View

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Birnboim H, DOLY J . A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979; 7(6):1513-23. PMC: 342324. DOI: 10.1093/nar/7.6.1513. View

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

Tanner A, Haffer C, Bratthall G, Visconti R, Socransky S . A study of the bacteria associated with advancing periodontitis in man. J Clin Periodontol. 1979; 6(5):278-307. DOI: 10.1111/j.1600-051x.1979.tb01931.x. View

Progulske A, Holt S . Transmission-scanning electron microscopic observations of selected Eikenella corrodens strains. J Bacteriol. 1980; 143(2):1003-18. PMC: 294406. DOI: 10.1128/jb.143.2.1003-1018.1980. View

10.

Hull R, GILL R, Hsu P, Minshew B, Falkow S . Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate. Infect Immun. 1981; 33(3):933-8. PMC: 350799. DOI: 10.1128/iai.33.3.933-938.1981. View

11.

Gold L, Pribnow D, Schneider T, Shinedling S, Singer B, Stormo G . Translational initiation in prokaryotes. Annu Rev Microbiol. 1981; 35:365-403. DOI: 10.1146/annurev.mi.35.100181.002053. View

12.

McKern N, ODONNELL I, Inglis A, Stewart D, Clark B . Amino acid sequence of pilin from Bacteroides nodosus (strain 198), the causative organism of ovine footrot. FEBS Lett. 1983; 164(1):149-53. DOI: 10.1016/0014-5793(83)80039-8. View

13.

Meyer T, Billyard E, Haas R, Storzbach S, So M . Pilus genes of Neisseria gonorrheae: chromosomal organization and DNA sequence. Proc Natl Acad Sci U S A. 1984; 81(19):6110-4. PMC: 391869. DOI: 10.1073/pnas.81.19.6110. View

14.

Elleman T, Hoyne P . Nucleotide sequence of the gene encoding pilin of Bacteroides nodosus, the causal organism of ovine footrot. J Bacteriol. 1984; 160(3):1184-7. PMC: 215842. DOI: 10.1128/jb.160.3.1184-1187.1984. View

15.

Sastry P, Finlay B, Pasloske B, Paranchych W, Pearlstone J, Smillie L . Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO. J Bacteriol. 1985; 164(2):571-7. PMC: 214290. DOI: 10.1128/jb.164.2.571-577.1985. View

16.

Stoloff A, Gillies M . Infections with Eikenella corrodens in a general hospital: a report of 33 cases. Rev Infect Dis. 1986; 8(1):50-3. DOI: 10.1093/clinids/8.1.50. View

17.

Tonjum T, Hagen N, BOVRE K . Identification of Eikenella corrodens and Cardiobacterium hominis by genetic transformation. Acta Pathol Microbiol Immunol Scand B. 1985; 93(6):389-94. DOI: 10.1111/j.1699-0463.1985.tb02907.x. View

18.

Taylor R, Miller V, Furlong D, Mekalanos J . Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin. Proc Natl Acad Sci U S A. 1987; 84(9):2833-7. PMC: 304754. DOI: 10.1073/pnas.84.9.2833. View

19.

Nicolson I, Perry A, Virji M, Heckels J, Saunders J . Localization of antibody-binding sites by sequence analysis of cloned pilin genes from Neisseria gonorrhoeae. J Gen Microbiol. 1987; 133(4):825-33. DOI: 10.1099/00221287-133-4-825. View

20.

Dalrymple B, Mattick J . An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins. J Mol Evol. 1987; 25(3):261-9. DOI: 10.1007/BF02100020. View