Transmission-scanning Electron Microscopic Observations of Selected Eikenella Corrodens Strains

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Aug 1

PMID 7009539

Citations 5

Authors

A Progulske

S C Holt

Affiliations

Soon will be listed here.

Abstract

The morphology of Eikenella corrodens 333/54-55 (ATCC 23834) and two human periodontal lesion isolates, strains 470 and 373, was examined by transmission and scanning electron microscopy. All strains exhibited a cell envelope characteristic of gram-negative bacteria. Staining with ruthenium red and alcian blue revealed a loosely organized fibrous slime layer associated with the outer surface of the outer membrane. Slime "stabilization" was achieved by incubation of cells with antisera prepared against whole cells of the Eikenella strains. The stabilized slime appeared as a thick, electron-opaque layer juxtaposed to the outer membrane. Negative staining and heavy metal shadow-casting revealed an interwoven network of fibrils approximately 4 nm in diameter. These fibrils appeared to represent subunits of a larger fibril. Scanning electron microscopy after antibody slime stabilization confirmed the presence and location of the slime layer.

Citing Articles

Eikenella corrodens phase variation involves a posttranslational event in pilus formation.

Villar M, Helber J, Hood B, Schaefer M, Hirschberg R J Bacteriol. 1999; 181(14):4154-60.

PMID: 10400570 PMC: 93914. DOI: 10.1128/JB.181.14.4154-4160.1999.

Sequence divergence in two tandemly located pilin genes of Eikenella corrodens.

Tonjum T, Weir S, BOVRE K, Progulske-Fox A, Marrs C Infect Immun. 1993; 61(5):1909-16.

PMID: 8478080 PMC: 280783. DOI: 10.1128/iai.61.5.1909-1916.1993.

Purification and characterization of Eikenella corrodens type IV pilin.

Hood B, HIRSCHBERG R Infect Immun. 1995; 63(9):3693-6.

PMID: 7642307 PMC: 173511. DOI: 10.1128/iai.63.9.3693-3696.1995.

Morphology and ultrastructure of oral strains of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus.

Holt S, Tanner A, Socransky S Infect Immun. 1980; 30(2):588-600.

PMID: 7439996 PMC: 551351. DOI: 10.1128/iai.30.2.588-600.1980.

Isolation and characterization of the outer membrane and lipopolysaccharide from Eikenella corrodens.

Progulske A, Holt S Infect Immun. 1984; 43(1):166-77.

PMID: 6360892 PMC: 263405. DOI: 10.1128/iai.43.1.166-177.1984.

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