Identification of Sequence Elements in Mouse Calbindin-D28k Gene That Confer 1,25-dihydroxyvitamin D3- and Butyrate-inducible Responses

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Apr 1

PMID 8464915

Citations 24

Authors

R K Gill

S Christakos

Affiliations

Soon will be listed here.

Abstract

We have examined the 5' flanking region of the mouse calbindin-D28k gene and identified a 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3]-responsive element by deletion mutant analysis of the native promoter as well as by studies with a heterologous thymidine kinase (TK) promoter. The segment between residues -200 and -169 was found to confer a dose-dependent 1,25-(OH)2D3 responsiveness through the TK promoter in Ros 17/2.8 cells as well as in CV-1 cells cotransfected with pAV-hVDR (human vitamin D receptor expression vector). This region contains sequences homologous to the rat osteocalcin vitamin D response element (VDRE). Incubation of this element with nuclear extracts from 1,25-(OH)2D3-treated Ros 17/2.8 cells or from 1,25-(OH)2D3-treated COS cells that had been transfected with pAV-hVDR resulted in a specific protein-DNA interaction. In addition to 1,25-(OH)2D3, sodium butyrate, a differentiating agent, has also been found to modulate expression of calbindin-D28k. Deletion analysis of the mouse calbindin-D28k promoter as well as studies with a heterologous TK promoter resulted in identification of a butyrate-responsive element between -180 and -150 that was found to bind specifically to nuclear factors from butyrate-treated Ros 17/2.8 cells. This butyrate-responsive element may represent a genetic element acted upon by enhancer binding proteins. In summary, the 5' flanking region of the mouse calbindin-D28k gene contains responsive elements that interact with nuclear factors and may mediate, at least in part, the enhanced expression of this gene by 1,25-(OH)2D3 and butyrate.

Citing Articles

Regulation of renal calbindin expression during cisplatin-induced kidney injury.

George B, Szilagyi J, Joy M, Aleksunes L J Biochem Mol Toxicol. 2022; 36(7):e23068.

PMID: 35403300 PMC: 9296602. DOI: 10.1002/jbt.23068.

Eldecalcitol is more effective in promoting osteogenesis than alfacalcidol in Cyp27b1-knockout mice.

Hirota Y, Nakagawa K, Isomoto K, Sakaki T, Kubodera N, Kamao M PLoS One. 2018; 13(10):e0199856.

PMID: 30281599 PMC: 6169848. DOI: 10.1371/journal.pone.0199856.

Isx9 Regulates Calbindin D28K Expression in Pancreatic β Cells and Promotes β Cell Survival and Function.

Pujol J, Heikkila E, Savoia C, Hajibeigi A, De Marchi U, Battiprolu P Int J Mol Sci. 2018; 19(9).

PMID: 30150605 PMC: 6165483. DOI: 10.3390/ijms19092542.

Modulation of renal calcium and phosphate transporting proteins by dietary nitrogen and/or calcium in young goats.

Firmenich C, Elfers K, Wilkens M, Breves G, Muscher-Banse A J Anim Sci. 2018; 96(8):3208-3220.

PMID: 29741700 PMC: 6095294. DOI: 10.1093/jas/sky185.

Biology and Mechanisms of Action of the Vitamin D Hormone.

Pike J, Christakos S Endocrinol Metab Clin North Am. 2017; 46(4):815-843.

PMID: 29080638 PMC: 5762112. DOI: 10.1016/j.ecl.2017.07.001.

References

Feinberg A, Vogelstein B . "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum. Anal Biochem. 1984; 137(1):266-7. DOI: 10.1016/0003-2697(84)90381-6. View

Opperman L, Saunders T, Bruns D, Boyd J, Mills S, Bruns M . Estrogen inhibits calbindin-D28k expression in mouse uterus. Endocrinology. 1992; 130(3):1728-35. DOI: 10.1210/endo.130.3.1537319. View

Ghosh M, Cox R . Production of human chorionic gonadotropin in HeLa cell cultures. Nature. 1976; 259(5542):416-7. DOI: 10.1038/259416a0. View

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

SANGER F, Nicklen S, Coulson A . DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977; 74(12):5463-7. PMC: 431765. DOI: 10.1073/pnas.74.12.5463. View

Majeska R, Rodan S, Rodan G . Parathyroid hormone-responsive clonal cell lines from rat osteosarcoma. Endocrinology. 1980; 107(5):1494-503. DOI: 10.1210/endo-107-5-1494. View

KRUH J . Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol Cell Biochem. 1982; 42(2):65-82. DOI: 10.1007/BF00222695. View

Dignam J, Lebovitz R, Roeder R . Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucleic Acids Res. 1983; 11(5):1475-89. PMC: 325809. DOI: 10.1093/nar/11.5.1475. View

Feher J . Facilitated calcium diffusion by intestinal calcium-binding protein. Am J Physiol. 1983; 244(3):C303-7. DOI: 10.1152/ajpcell.1983.244.3.C303. View

10.

Wood C, Tonegawa S . Diversity and joining segments of mouse immunoglobulin heavy chain genes are closely linked and in the same orientation: implications for the joining mechanism. Proc Natl Acad Sci U S A. 1983; 80(10):3030-4. PMC: 393967. DOI: 10.1073/pnas.80.10.3030. View

11.

Birren B, Herschman H . Regulation of the rat metallothionein-I gene by sodium butyrate. Nucleic Acids Res. 1986; 14(2):853-67. PMC: 339469. DOI: 10.1093/nar/14.2.853. View

12.

Yamakuni T, Kuwano R, Odani S, Miki N, Yamaguchi Y, Takahashi Y . Nucleotide sequence of cDNA to mRNA for a cerebellar Ca-binding protein, spot 35 protein. Nucleic Acids Res. 1986; 14(16):6768. PMC: 311681. DOI: 10.1093/nar/14.16.6768. View

13.

Theofan G, Nguyen A, Norman A . Regulation of calbindin-D28K gene expression by 1,25-dihydroxyvitamin D3 is correlated to receptor occupancy. J Biol Chem. 1986; 261(36):16943-7. View

14.

Philippe J, Drucker D, Chick W, Habener J . Transcriptional regulation of genes encoding insulin, glucagon, and angiotensinogen by sodium butyrate in a rat islet cell line. Mol Cell Biol. 1987; 7(1):560-3. PMC: 365105. DOI: 10.1128/mcb.7.1.560-563.1987. View

15.

Dupret J, Brun P, Perret C, Lomri N, Thomasset M, CUISINIER-GLEIZES P . Transcriptional and post-transcriptional regulation of vitamin D-dependent calcium-binding protein gene expression in the rat duodenum by 1,25-dihydroxycholecalciferol. J Biol Chem. 1987; 262(34):16553-7. View

16.

Bronner F, Stein W . CaBPr facilitates intracellular diffusion for Ca pumping in distal convoluted tubule. Am J Physiol. 1988; 255(3 Pt 2):F558-62. DOI: 10.1152/ajprenal.1988.255.3.F558. View

17.

Hunziker W, Schrickel S . Rat brain calbindin D28: six domain structure and extensive amino acid homology with chicken calbindin D28. Mol Endocrinol. 1988; 2(5):465-73. DOI: 10.1210/mend-2-5-465. View

18.

Demay M, Roth D, Kronenberg H . Regions of the rat osteocalcin gene which mediate the effect of 1,25-dihydroxyvitamin D3 on gene transcription. J Biol Chem. 1989; 264(4):2279-82. View

19.

Wood T, Kobayashi Y, Frantz G, Varghese S, Christakos S, Tobin A . Molecular cloning of mammalian 28,000 Mr vitamin D-dependent calcium binding protein (calbindin-D28K): expression of calbindin-D28K RNAs in rodent brain and kidney. DNA. 1988; 7(9):585-93. DOI: 10.1089/dna.1988.7.585. View

20.

McDonnell D, Scott R, Kerner S, OMalley B, Pike J . Functional domains of the human vitamin D3 receptor regulate osteocalcin gene expression. Mol Endocrinol. 1989; 3(4):635-44. DOI: 10.1210/mend-3-4-635. View