Identification, Characterization and Cloning of Myr 1, a Mammalian Myosin-I

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1993 Mar 1

PMID 8449985

Citations 38

Authors

C Ruppert

R Kroschewski

M Bahler

Affiliations

Soon will be listed here.

Abstract

We have identified, characterized and cloned a novel mammalian myosin-I motor-molecule, called myr 1 (myosin-I from rat). Myr 1 exists in three alternative splice forms: myr 1a, myr 1b, and myr 1c. These splice forms differ in their numbers of putative calmodulin/light chain binding sites. Myr 1a-c were selectively released by ATP, bound in a nucleotide-dependent manner to F-actin and exhibited amino acid sequences characteristic of myosin-I motor domains. In addition to the motor domain, they contained a regulatory domain with up to six putative calmodulin/light chain binding sites and a tail domain. The tail domain exhibited 47% amino acid sequence identity to the brush border myosin-I tail domain, demonstrating that myr 1 is related to the only other mammalian myosin-I motor molecule that has been characterized so far. In contrast to brush border myosin-I which is expressed in mature enterocytes, myr 1 splice forms were differentially expressed in all tested tissues. Therefore, myr 1 is the first mammalian myosin-I motor molecule with a widespread tissue distribution in neonatal and adult tissues. The myr 1a splice form was preferentially expressed in neuronal tissues. Its expression was developmentally regulated during rat forebrain ontogeny and subcellular fractionation revealed an enrichment in purified growth cone particles, data consistent with a role for myr 1a in neuronal development.

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References

Fukui Y, Lynch T, Brzeska H, Korn E . Myosin I is located at the leading edges of locomoting Dictyostelium amoebae. Nature. 1989; 341(6240):328-31. DOI: 10.1038/341328a0. View

Mitchison T, Kirschner M . Cytoskeletal dynamics and nerve growth. Neuron. 1988; 1(9):761-72. DOI: 10.1016/0896-6273(88)90124-9. View

Cleveland D, Fischer S, Kirschner M, Laemmli U . Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977; 252(3):1102-6. View

Coluccio L, BRETSCHER A . Reassociation of microvillar core proteins: making a microvillar core in vitro. J Cell Biol. 1989; 108(2):495-502. PMC: 2115419. DOI: 10.1083/jcb.108.2.495. View

Glenney Jr J, Weber K . Calmodulin-binding proteins of the microfilaments present in isolated brush borders and microvilli of intestinal epithelial cells. J Biol Chem. 1980; 255(22):10551-4. View

Barylko B, Wagner M, Reizes O, Albanesi J . Purification and characterization of a mammalian myosin I. Proc Natl Acad Sci U S A. 1992; 89(2):490-4. PMC: 48264. DOI: 10.1073/pnas.89.2.490. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Mornet D, Bonet A, Audemard E, Bonicel J . Functional sequences of the myosin head. J Muscle Res Cell Motil. 1989; 10(1):10-24. DOI: 10.1007/BF01739853. View

Green N, Alexander H, Olson A, Alexander S, Shinnick T, Sutcliffe J . Immunogenic structure of the influenza virus hemagglutinin. Cell. 1982; 28(3):477-87. DOI: 10.1016/0092-8674(82)90202-1. View

10.

Matsudaira P, Burgess D . Identification and organization of the components in the isolated microvillus cytoskeleton. J Cell Biol. 1979; 83(3):667-73. PMC: 2110511. DOI: 10.1083/jcb.83.3.667. View

11.

Jung G, Saxe 3rd C, Kimmel A, Hammer 3rd J . Dictyostelium discoideum contains a gene encoding a myosin I heavy chain. Proc Natl Acad Sci U S A. 1989; 86(16):6186-90. PMC: 297802. DOI: 10.1073/pnas.86.16.6186. View

12.

Toyoshima Y, Kron S, MCNALLY E, Niebling K, Toyoshima C, Spudich J . Myosin subfragment-1 is sufficient to move actin filaments in vitro. Nature. 1987; 328(6130):536-9. DOI: 10.1038/328536a0. View

13.

Hayden S, Wolenski J, Mooseker M . Binding of brush border myosin I to phospholipid vesicles. J Cell Biol. 1990; 111(2):443-51. PMC: 2116197. DOI: 10.1083/jcb.111.2.443. View

14.

Sbaschnig-Agler M, Pfenninger K, Ledeen R . Gangliosides and other lipids of the growth cone membrane. J Neurochem. 1988; 51(1):212-20. DOI: 10.1111/j.1471-4159.1988.tb04858.x. View

15.

Harada Y, Noguchi A, Kishino A, Yanagida T . Sliding movement of single actin filaments on one-headed myosin filaments. Nature. 1987; 326(6115):805-8. DOI: 10.1038/326805a0. View

16.

Adams R, Pollard T . Propulsion of organelles isolated from Acanthamoeba along actin filaments by myosin-I. Nature. 1986; 322(6081):754-6. DOI: 10.1038/322754a0. View

17.

Pollard T, Doberstein S, Zot H . Myosin-I. Annu Rev Physiol. 1991; 53:653-81. DOI: 10.1146/annurev.ph.53.030191.003253. View

18.

Pardee J, Spudich J . Purification of muscle actin. Methods Cell Biol. 1982; 24:271-89. DOI: 10.1016/s0091-679x(08)60661-5. View

19.

Pollard T, Korn E . Acanthamoeba myosin. I. Isolation from Acanthamoeba castellanii of an enzyme similar to muscle myosin. J Biol Chem. 1973; 248(13):4682-90. View

20.

Kawakami H, Moriyoshi K, Utsumi T, Nakanishi S . Structural organization and expression of the gene for bovine myosin I heavy chain. J Biochem. 1992; 111(3):302-9. DOI: 10.1093/oxfordjournals.jbchem.a123754. View