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Fast DNA Isolation from Histoplasma Capsulatum: Methodology for Arbitrary Primer Polymerase Chain Reaction-based Epidemiological and Clinical Studies

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1993 Feb 1
PMID 8432840
Citations 23
Authors
J P Woods
D Kersulyte
W E Goldman
D E Berg
Affiliations
Soon will be listed here.
Abstract

The arbitrary primer polymerase chain reaction (also called random amplified polymorphic DNA, or RAPD) is a DNA fingerprinting method that provides an efficient, sensitive way of discriminating between independent isolates of Histoplasma capsulatum, but its widespread application has been hampered by the arduous 2-day procedure traditionally used to extract DNA from H. capsulatum. We present here a quick (approximately 2-h) extraction method and show that the resultant DNA is suitable for sensitive and reproducible identification of individual strains of this pathogenic fungus.

Citing Articles

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Selection of Polymorphic Patterns Obtained by RAPD-PCR through Qualitative and Quantitative Analyses to Differentiate .

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Analyses of the genetic diversity and population structures of Histoplasma capsulatum clinical isolates from Mexico, Guatemala, Colombia and Argentina, using a randomly amplified polymorphic DNA-PCR assay.

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The use of genetic markers in the molecular epidemiology of histoplasmosis: a systematic review.

Damasceno L, Leitao T, Taylor M, Muniz M, Zancope-Oliveira R Eur J Clin Microbiol Infect Dis. 2015; 35(1):19-27.

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Rapid identification of Sporothrix species by T3B fingerprinting.

Evangelista de Oliveira M, Sampaio P, Almeida-Paes R, Pais C, Gutierrez-Galhardo M, Zancope-Oliveira R J Clin Microbiol. 2012; 50(6):2159-62.

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References
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