The Identification of Y Chromosome-linked Markers with Random Sequence Oligonucleotide Primers

Overview

Journal Mamm Genome

Specialty Genetics

Date 1993 Jan 1

PMID 8431634

Citations 4

Authors

B B Wardell

J D Sudweeks

N D Meeker

S S Estes

S R Woodward

C Teuscher

Affiliations

Soon will be listed here.

Abstract

The polymerase chain reaction (PCR)-based technique of random amplification of polymorphic DNA (RAPD) is extremely useful for developing DNA-based markers. We previously identified a linkage group of eight unmapped RAPD markers that distinguish C57BL/6J and DBA/2J mice (Mammalian Genome 3: Woodward et al., 73-78, 1992). In this study, we report that all eight markers are Y Chromosome (Chr)-linked. One additional Y-linked RAPD was discovered serendipitously during the screening of a C3H/HeJ x (C3H/HeJ x SJL/J)F1 BC1 population. The segregation of all nine markers was analyzed with a panel of 14 independent inbred strains of male mice. The nine markers could be divided into three distinct groups: (1) DYByu2, DYByu5, DYByu6, and DYByu8 identify both the M.m. musculus and M.m. domesticus type Y Chr; (2) DYByu1, DYByu3, DYByu4, and DYByu7 are specific for the M.m. musculus type; and (3) DYByu9 is specific for the M.m. domesticus type. The results clearly indicate that the RAPD technique can be used to identify Y Chr-linked, DNA-based markers in mammalian species.

Citing Articles

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Identification and genetic mapping of random amplified polymorphic DNA (RAPD) markers to the sheep genome.

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Fine genetic mapping of the region surrounding the high growth (hg) locus in mouse chromosome 10: targeting random amplified polymorphic DNA (RAPD) markers.

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