Low-frequency Chimeric Yeast Artificial Chromosome Libraries from Flow-sorted Human Chromosomes 16 and 21

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Feb 1

PMID 8430075

Citations 13

Authors

M K McCormick

E Campbell

L Deaven

R Moyzis

Affiliations

Soon will be listed here.

Abstract

Construction of chromosome-specific yeast artificial chromosome (YAC) libraries from sorted chromosomes was undertaken (i) to eliminate drawbacks associated with first-generation total genomic YAC libraries, such as the high frequency of chimeric YACs, and (ii) to provide an alternative method for generating chromosome-specific YAC libraries in addition to isolating such collections from a total genomic library. Chromosome-specific YAC libraries highly enriched for human chromosomes 16 and 21 were constructed. By maximizing the percentage of fragments with two ligatable ends and performing yeast transformations with less than saturating amounts of DNA in the presence of carrier DNA, YAC libraries with a low percentage of chimeric clones were obtained. The smaller number of YAC clones in these chromosome-specific libraries reduces the effort involved in PCR-based screening and allows hybridization methods to be a manageable screening approach.

Citing Articles

High-resolution flow karyotyping and chromosome sorting in Vicia faba lines with standard and reconstructed karyotypes.

Dolezel J, Lucretti S Theor Appl Genet. 2013; 90(6):797-802.

PMID: 24172921 DOI: 10.1007/BF00222014.

Chromosomes in the flow to simplify genome analysis.

Dolezel J, Vrana J, Safar J, Bartos J, Kubalakova M, Simkova H Funct Integr Genomics. 2012; 12(3):397-416.

PMID: 22895700 PMC: 3431466. DOI: 10.1007/s10142-012-0293-0.

Flow karyotypes and chromosomal DNA contents of genus Triticum species and rye (Secale cereale).

Lee J, Ma Y, Wako T, Li L, Kim K, Park S Chromosome Res. 2004; 12(1):93-102.

PMID: 14984105 DOI: 10.1023/b:chro.0000009327.45035.84.

Flow sorting of mitotic chromosomes in common wheat (Triticum aestivum L.).

Vrana J, Kubalakova M, Simkova H, Cihalikova J, Lysak M, Dolezel J Genetics. 2000; 156(4):2033-41.

PMID: 11102393 PMC: 1461381. DOI: 10.1093/genetics/156.4.2033.

Flow karyotyping and sorting of mitotic chromosomes of barley (Hordeum vulgare L.).

Lysak M, Cihalikova J, Kubalakova M, Simkova H, Kunzel G, Dolezel J Chromosome Res. 1999; 7(6):431-44.

PMID: 10560966 DOI: 10.1023/a:1009293628638.

References

Kozak C, Lawrence J, Ruddle F . A sequential staining technique for the chromosomal analysis of the interspecific mouse/hamster and mouse/human somatic cell hybrids. Exp Cell Res. 1977; 105(1):109-17. DOI: 10.1016/0014-4827(77)90156-2. View

Chumakov I, Rigault P, Guillou S, Ougen P, Billaut A, Guasconi G . Continuum of overlapping clones spanning the entire human chromosome 21q. Nature. 1992; 359(6394):380-7. DOI: 10.1038/359380a0. View

Deaven L, Van Dilla M, Bartholdi M, Carrano A, Cram L, Fuscoe J . Construction of human chromosome-specific DNA libraries from flow-sorted chromosomes. Cold Spring Harb Symp Quant Biol. 1986; 51 Pt 1:159-67. DOI: 10.1101/sqb.1986.051.01.019. View

Burgers P, Percival K . Transformation of yeast spheroplasts without cell fusion. Anal Biochem. 1987; 163(2):391-7. DOI: 10.1016/0003-2697(87)90240-5. View

Bartholdi M, Meyne J, Albright K, Luedemann M, Campbell E, Chritton D . Chromosome sorting by flow cytometry. Methods Enzymol. 1987; 151:252-67. DOI: 10.1016/s0076-6879(87)51022-9. View

LITTLE R, Porta G, Carle G, Schlessinger D, DUrso M . Yeast artificial chromosomes with 200- to 800-kilobase inserts of human DNA containing HLA, V kappa, 5S, and Xq24-Xq28 sequences. Proc Natl Acad Sci U S A. 1989; 86(5):1598-602. PMC: 286745. DOI: 10.1073/pnas.86.5.1598. View

Riethman H, Moyzis R, Meyne J, Burke D, Olson M . Cloning human telomeric DNA fragments into Saccharomyces cerevisiae using a yeast-artificial-chromosome vector. Proc Natl Acad Sci U S A. 1989; 86(16):6240-4. PMC: 297813. DOI: 10.1073/pnas.86.16.6240. View

Nelson D, Ledbetter S, Corbo L, Victoria M, Ramirez-Solis R, Webster T . Alu polymerase chain reaction: a method for rapid isolation of human-specific sequences from complex DNA sources. Proc Natl Acad Sci U S A. 1989; 86(17):6686-90. PMC: 297910. DOI: 10.1073/pnas.86.17.6686. View

McCormick M, Shero J, Cheung M, Kan Y, Hieter P, Antonarakis S . Construction of human chromosome 21-specific yeast artificial chromosomes. Proc Natl Acad Sci U S A. 1989; 86(24):9991-5. PMC: 298628. DOI: 10.1073/pnas.86.24.9991. View

10.

Wada M, LITTLE R, Abidi F, Porta G, Labella T, Cooper T . Human Xq24-Xq28: approaches to mapping with yeast artificial chromosomes. Am J Hum Genet. 1990; 46(1):95-106. PMC: 1683533. View

11.

Green E, Olson M . Systematic screening of yeast artificial-chromosome libraries by use of the polymerase chain reaction. Proc Natl Acad Sci U S A. 1990; 87(3):1213-7. PMC: 53441. DOI: 10.1073/pnas.87.3.1213. View

12.

Van Dilla M, Deaven L . Construction of gene libraries for each human chromosome. Cytometry. 1990; 11(1):208-18. DOI: 10.1002/cyto.990110124. View

13.

Abidi F, Wada M, LITTLE R, Schlessinger D . Yeast artificial chromosomes containing human Xq24-Xq28 DNA: library construction and representation of probe sequences. Genomics. 1990; 7(3):363-76. DOI: 10.1016/0888-7543(90)90170-y. View

14.

Stallings R, Torney D, Hildebrand C, Longmire J, Deaven L, Jett J . Physical mapping of human chromosomes by repetitive sequence fingerprinting. Proc Natl Acad Sci U S A. 1990; 87(16):6218-22. PMC: 54504. DOI: 10.1073/pnas.87.16.6218. View

15.

Bronson S, Pei J, Taillon-Miller P, Chorney M, Geraghty D, Chaplin D . Isolation and characterization of yeast artificial chromosome clones linking the HLA-B and HLA-C loci. Proc Natl Acad Sci U S A. 1991; 88(5):1676-80. PMC: 51087. DOI: 10.1073/pnas.88.5.1676. View

16.

Connelly C, McCormick M, Shero J, Hieter P . Polyamines eliminate an extreme size bias against transformation of large yeast artificial chromosome DNA. Genomics. 1991; 10(1):10-6. DOI: 10.1016/0888-7543(91)90477-v. View

17.

Shero J, McCormick M, Antonarakis S, Hieter P . Yeast artificial chromosome vectors for efficient clone manipulation and mapping. Genomics. 1991; 10(2):505-8. DOI: 10.1016/0888-7543(91)90343-d. View

18.

Green E, Riethman H, Dutchik J, Olson M . Detection and characterization of chimeric yeast artificial-chromosome clones. Genomics. 1991; 11(3):658-69. DOI: 10.1016/0888-7543(91)90073-n. View

19.

Hieter P, Mann C, Snyder M, Davis R . Mitotic stability of yeast chromosomes: a colony color assay that measures nondisjunction and chromosome loss. Cell. 1985; 40(2):381-92. DOI: 10.1016/0092-8674(85)90152-7. View