T Cell Redistribution Kinetics After Secondary Infection of BALB/c Mice with Respiratory Syncytial Virus

BALB/c mice were infected intranasally with live respiratory syncytial virus (RSV) and reinfected 4 weeks later. At regular intervals thereafter groups of animals were killed and T cell subsets were determined in blood, spleen and bronchoalveolar lavage (BAL) with flow cytometry employing T cell subset-specific MoAbs. Total lymphocyte counts in the peripheral blood decreased 1-3 days after infection, returning to preinfection levels on day 8 (P = 0.0111). Simultaneously, a marked increase of lymphocytes was noted in the BAL, reaching a maximum at day 8 (P < 0.0001). Both CD4+ and CD8+ T cells decreased in the blood on day 1-3 (P < 0.0097 and P = 0.003 respectively), and increased in the BAL progressively towards a maximum at day 8 (P < 0.0001). In BAL, CD4+ cells increased 35-fold and CD8+ cells 27-fold during the first week after reinfection. On the other hand, in the spleen a significant decline of CD4+ and CD8+ cells was noted 1 day post-infection (P = 0.0002). It is concluded that a strong T cell redistribution response among systemic and mucosal tissues occurs after reinfection with RSV. The kinetics of this response differ both quantitatively and qualitatively from the T cell response after primary infection. The magnitude of cell traffic is more pronounced in blood, spleen and BAL than after primary infection. CD4+ T cells are more intensively distributed to the lungs than after primary infection.