Serum Contains a Potent Factor That Decreases Beta-adrenergic Receptor-stimulated L-type Ca2+ Current in Cardiac Myocytes

Overview

Journal Pflugers Arch

Specialty Physiology

Date 1993 May 1

PMID 8391681

Citations 5

Authors

K Banach

M Bunemann

J Huser

L Pott

Affiliations

Soon will be listed here.

Abstract

L-type Ca2+ current (ICa) was measured in cultured atrial myocytes from hearts of adult guinea-pigs using whole-cell voltage clamp. Potentiation of ICa induced by beta-adrenergic stimulation (isoprenaline 2.10(-7) M) could be completely antagonized by diluted sera (1:100 v/v). Half-maximal inhibition of beta-receptor-stimulated ICa occurred at about 1:1000. Basal ICa was not affected by serum. Atropine in a concentration (10(-6) M) that completely antagonized the anti-adrenergic effect of acetylcholine (ACh, 2.10(-6) M) did not interfere with the effect of serum. In cells dialysed with cyclic adenosine monophosphate (cAMP)-containing (10(-4) M) pipette solution, potentiated ICa was insensitive to both ACh and serum. Preincubation of the myocytes with pertussis toxin almost completely abolished the anti-adrenergic effects of both ACh and serum. The potency of serum was not reduced by dialysis. It is concluded that serum contains a factor which, like ACh, inhibits beta-receptor-stimulated adenylyl cyclase via Gi-protein.

Citing Articles

Sphingosylphosphocholine is a naturally occurring lipid mediator in blood plasma: a possible role in regulating cardiac function via sphingolipid receptors.

Liliom K, Sun G, Bunemann M, Virag T, Nusser N, Baker D Biochem J. 2001; 355(Pt 1):189-97.

PMID: 11256963 PMC: 1221726. DOI: 10.1042/0264-6021:3550189.

A novel membrane receptor with high affinity for lysosphingomyelin and sphingosine 1-phosphate in atrial myocytes.

Bunemann M, Liliom K, Brandts B, Pott L, Tseng J, Desiderio D EMBO J. 1996; 15(20):5527-34.

PMID: 8896446 PMC: 452297.

Downregulation of muscarinic M2 receptors linked to K+ current in cultured guinea-pig atrial myocytes.

Bunemann M, Brandts B, Pott L J Physiol. 1996; 494 ( Pt 2):351-62.

PMID: 8841996 PMC: 1160639. DOI: 10.1113/jphysiol.1996.sp021497.

Activation of muscarinic K+ current in guinea-pig atrial myocytes by sphingosine-1-phosphate.

Bunemann M, Brandts B, zu Heringdorf D, van Koppen C, Jakobs K, Pott L J Physiol. 1995; 489 ( Pt 3):701-7.

PMID: 8788935 PMC: 1156840. DOI: 10.1113/jphysiol.1995.sp021084.

Membrane-delimited activation of muscarinic K current by an albumin-associated factor in guinea-pig atrial myocytes.

Bunemann M, Pott L Pflugers Arch. 1993; 425(3-4):329-34.

PMID: 8309794 DOI: 10.1007/BF00374183.

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