A Mutation in PLC1, a Candidate Phosphoinositide-specific Phospholipase C Gene from Saccharomyces Cerevisiae, Causes Aberrant Mitotic Chromosome Segregation

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1993 Jul 1

PMID 8391635

Citations 35

Authors

W E Payne

M Fitzgerald-Hayes

Affiliations

Soon will be listed here.

Abstract

We identified a putative Saccharomyces cerevisiae homolog of a phosphoinositide-specific phospholipase C (PI-PLC) gene, PLC1, which encodes a protein most similar to the delta class of PI-PLC enzymes. The PLC1 gene was isolated during a study of yeast strains that exhibit defects in chromosome segregation. plc1-1 cells showed a 10-fold increase in aberrant chromosome segregation compared with the wild type. Molecular analysis revealed that PLC1 encodes a predicted protein of 101 kDa with approximately 50 and 26% identity to the highly conserved X and Y domains of PI-PLC isozymes from humans, bovines, rats, and Drosophila melanogaster. The putative yeast protein also contains a consensus EF-hand domain that is predicted to bind calcium. Interestingly, the temperature-sensitive and chromosome missegregation phenotypes exhibited by plc1-1 cells were partially suppressed by exogenous calcium.

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