Functional Binding of Cardiolipin to Cytochrome C Oxidase

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1993 Apr 1

PMID 8389748

Citations 77

Authors

N C Robinson

Affiliations

Soon will be listed here.

Abstract

Bovine cytochrome c oxidase usually contains 3-4 mol of tightly bound cardiolipin per cytochrome aa3 complex. At least two of these cardiolipins are required for full electron transport activity. Without the tightly bound cardiolipin, cytochrome c oxidase has only 40-50% of its original activity when assayed in detergents that support activity, e.g., dodecyl maltoside. By measuring the restoration of electron transport activity, functional binding constants for cardiolipin and a number of cardiolipin analogues have been evaluated (Kd,app = 1 microM for cardiolipin). These binding constants agree reasonably well with direct measurement of the binding using [14C]-acetyl-cardiolipin (Kd < 0.1 microM) when the enzyme is solubilized with Triton X-100. These data are discussed in relationship to the wealth of data that is known about the association of cardiolipin with cytochrome c oxidase and the other mitochondrial electron transport complexes and transporters.

Citing Articles

Cardiolipin deficiency disrupts CoQ-complex III interface in steatohepatitis.

Brothwell M, Cao G, Maschek J, Poss A, Peterlin A, Wang L bioRxiv. 2024; .

PMID: 39416056 PMC: 11482932. DOI: 10.1101/2024.10.10.617517.

Age-dependent loss of Crls1 causes myopathy and skeletal muscle regeneration failure.

Yoo Y, Yeon M, Kim W, Shin H, Lee S, Yoon M Exp Mol Med. 2024; 56(4):922-934.

PMID: 38556544 PMC: 11059380. DOI: 10.1038/s12276-024-01199-x.

Genetic deletion of skeletal muscle iPLAγ results in mitochondrial dysfunction, muscle atrophy and alterations in whole-body energy metabolism.

Moon S, Dilthey B, Guan S, Sims H, Pittman S, Keith A iScience. 2023; 26(6):106895.

PMID: 37275531 PMC: 10239068. DOI: 10.1016/j.isci.2023.106895.

Advances in methods to analyse cardiolipin and their clinical applications.

Bautista J, Falabella M, Flannery P, Hanna M, Heales S, Pope S Trends Analyt Chem. 2023; 157:116808.

PMID: 36751553 PMC: 7614147. DOI: 10.1016/j.trac.2022.116808.

Physiological levels of cardiolipin acutely affect mitochondrial respiration in vascular smooth muscle cells.

Galambo D, Bergdahl A Curr Res Physiol. 2023; 6:100097.

PMID: 36594049 PMC: 9803913. DOI: 10.1016/j.crphys.2022.100097.

References

Nicolay K, Timmers R, Spoelstra E, van der Neut R, Fok J, Huigen Y . The interaction of adriamycin with cardiolipin in model and rat liver mitochondrial membranes. Biochim Biophys Acta. 1984; 778(2):359-71. DOI: 10.1016/0005-2736(84)90380-8. View

Beleznai Z, Jancsik V . Role of cardiolipin in the functioning of mitochondrial L-glycerol-3-phosphate dehydrogenase. Biochem Biophys Res Commun. 1989; 159(1):132-9. DOI: 10.1016/0006-291x(89)92414-5. View

Jones M, Rashid K, Wilson M . An active cytochrome c oxidase that has no tightly bound cardiolipin. Biochem J. 1983; 209(3):901-3. PMC: 1154174. DOI: 10.1042/bj2090901. View

Jones M, Wilson M . The phospholipids associated with cytochrome c oxidase isolated from beef, dogfish and cod heart. Comp Biochem Physiol B. 1984; 77(3):609-16. DOI: 10.1016/0305-0491(84)90284-0. View

Goormaghtigh E, Brasseur R, Ruysschaert J . Adriamycin inactivates cytochrome c oxidase by exclusion of the enzyme from its cardiolipin essential environment. Biochem Biophys Res Commun. 1982; 104(1):314-20. DOI: 10.1016/0006-291x(82)91976-3. View

Demant E . NADH oxidation in submitochondrial particles protects respiratory chain activity against damage by adriamycin-Fe3+. Eur J Biochem. 1983; 137(1-2):113-8. DOI: 10.1111/j.1432-1033.1983.tb07803.x. View

Powell G, Knowles P, Marsh D . Association of spin-labelled cardiolipin with dimyristoylphosphatidylcholine-substituted bovine heart cytochrome c oxidase. A generalized specificity increase rather than highly specific binding sites. Biochim Biophys Acta. 1985; 816(1):191-4. DOI: 10.1016/0005-2736(85)90409-2. View

Cheneval D, Carafoli E . Identification and primary structure of the cardiolipin-binding domain of mitochondrial creatine kinase. Eur J Biochem. 1988; 171(1-2):1-9. DOI: 10.1111/j.1432-1033.1988.tb13750.x. View

Noel H, PANDE S . An essential requirement of cardiolipin for mitochondrial carnitine acylcarnitine translocase activity. Lipid requirement of carnitine acylcarnitine translocase. Eur J Biochem. 1986; 155(1):99-102. DOI: 10.1111/j.1432-1033.1986.tb09463.x. View

10.

Kramer R, Klingenberg M . Enhancement of reconstituted ADP,ATP exchange activity by phosphatidylethanolamine and by anionic phospholipids. FEBS Lett. 1980; 119(2):257-60. DOI: 10.1016/0014-5793(80)80266-3. View

11.

Robinson N, Capaldi R . Interaction of detergents with cytochrome c oxidase. Biochemistry. 1977; 16(3):375-81. DOI: 10.1021/bi00622a006. View

12.

Hill B, Cook K, Robinson N . Subunit dissociation and protein unfolding in the bovine heart cytochrome oxidase complex induced by guanidine hydrochloride. Biochemistry. 1988; 27(13):4741-7. DOI: 10.1021/bi00413a024. View

13.

Cheneval D, Muller M, Toni R, Ruetz S, Carafoli E . Adriamycin as a probe for the transversal distribution of cardiolipin in the inner mitochondrial membrane. J Biol Chem. 1985; 260(24):13003-7. View

14.

Mende P, Huther F, KADENBACH B . Specific and reversible activation and inactivation of the mitochondrial phosphate carrier by cardiolipin and nonionic detergents, respectively. FEBS Lett. 1983; 158(2):331-4. DOI: 10.1016/0014-5793(83)80607-3. View

15.

Ioannou P, Golding B . Cardiolipins: their chemistry and biochemistry. Prog Lipid Res. 1979; 17(3):279-318. DOI: 10.1016/0079-6832(79)90010-7. View

16.

Goormaghtigh E, Chatelain P, Caspers J, Ruysschaert J . Evidence of a specific complex between adriamycin and negatively-charged phospholipids. Biochim Biophys Acta. 1980; 597(1):1-14. DOI: 10.1016/0005-2736(80)90145-5. View

17.

Goormaghtigh E, Huart P, Brasseur R, Ruysschaert J . Mechanism of inhibition of mitochondrial enzymatic complex I-III by adriamycin derivatives. Biochim Biophys Acta. 1986; 861(1):83-94. DOI: 10.1016/0005-2736(86)90374-3. View

18.

Robinson N . Specificity and binding affinity of phospholipids to the high-affinity cardiolipin sites of beef heart cytochrome c oxidase. Biochemistry. 1982; 21(1):184-8. DOI: 10.1021/bi00530a031. View

19.

Cable M, Powell G . Spin-labeled cardiolipin: preferential segregation in the boundary layer of cytochrome c oxidase. Biochemistry. 1980; 19(25):5679-86. DOI: 10.1021/bi00566a003. View

20.

Awasthi Y, Chuang T, Keenan T, Crane F . Tightly bound cardiolipin in cytochrome oxidase. Biochim Biophys Acta. 1971; 226(1):42-52. DOI: 10.1016/0005-2728(71)90176-9. View