Analysis of Antigen Receptor Genes in Hodgkin's Disease

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1993 Apr 1

PMID 8388407

Citations 4

Authors

C A Angel

J H Pringle

J Naylor

K P West

I LAUDER

Affiliations

Soon will be listed here.

Abstract

Aim: To analyse the configuration of the antigen receptor genes in Hodgkin's disease.

Methods: DNA extracted from 45 samples of Hodgkin's disease was analysed using Southern blotting and DNA hybridisation, using probes to the joining region of the immunoglobulin heavy chain gene, the constant region of kappa immunoglobulin light chain gene, and the constant region of the beta chain of the T cell receptor gene.

Results: A single case of nodular sclerosing disease showed clonal rearrangement of the immunoglobulin heavy and light chain genes, all other samples having germline immunoglobulin genes. The nature of the clonal population in the diseased tissue is uncertain, because the intensity of the rearranged bands did not correlate with the percentage of Reed-Sternberg cells present. The T cell receptor genes were in germline configuration in all the samples.

Conclusions: Antigen receptor gene rearrangement is a rare finding in unselected cases of Hodgkin's disease.

Citing Articles

PCR clonality detection in Hodgkin lymphoma.

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Expression of B7 (CD80) and CD40 antigens and the CD40 ligand in Hodgkin's disease is independent of latent Epstein-Barr virus infection.

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Angiotropic lymphoma: report of a case with histiocytic features.

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Detection of immunoglobulin heavy chain gene rearrangements in Hodgkin's disease using PCR.

Angel C, Pringle J, Primrose L, LAUDER I J Clin Pathol. 1993; 46(10):940-2.

PMID: 8227413 PMC: 501623. DOI: 10.1136/jcp.46.10.940.

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