Further Molecular Characterization of the Cloned Legionella Pneumophila Zinc Metalloprotease

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1994 Feb 1

PMID 8300238

Citations 7

Authors

J F Moffat

W J Black

L S Tompkins

Affiliations

Soon will be listed here.

Abstract

On the basis of DNA sequence similarities to other Zn metalloproteases, further studies of the synthesis, processing, and enzymatic structure of the cloned Legionella protease gene, proA, were initiated. TnphoA fusions indicated that the entire proA open reading frame was transcribed and translated, including the 5' leader sequence. The results also suggested that the entire polypeptide was exported to the periplasm before cleavage to produce the mature protease. A site-directed mutation in the putative active site, changing glutamate 378 to asparagine, abolished proteolytic activity and cytotoxicity.

Citing Articles

Protein sociology of ProA, Mip and other secreted virulence factors at the surface.

Scheithauer L, Karagoz M, Mayer B, Steinert M Front Cell Infect Microbiol. 2023; 13:1140688.

PMID: 36936764 PMC: 10017501. DOI: 10.3389/fcimb.2023.1140688.

Disulfide loop cleavage of Legionella pneumophila PlaA boosts lysophospholipase A activity.

Lang C, Hiller M, Flieger A Sci Rep. 2017; 7(1):16313.

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Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2.

Hirayasu K, Saito F, Suenaga T, Shida K, Arase N, Oikawa K Nat Microbiol. 2016; 1(6):16054.

PMID: 27572839 DOI: 10.1038/nmicrobiol.2016.54.

Zinc metalloproteinase ProA directly activates Legionella pneumophila PlaC glycerophospholipid:cholesterol acyltransferase.

Lang C, Rastew E, Hermes B, Siegbrecht E, Ahrends R, Banerji S J Biol Chem. 2012; 287(28):23464-78.

PMID: 22582391 PMC: 3390623. DOI: 10.1074/jbc.M112.346387.

Highly sensitive quenched fluorescent substrate of Legionella major secretory protein (msp) based on its structural analysis.

Poras H, Duquesnoy S, Dange E, Pinon A, Vialette M, Fournie-Zaluski M J Biol Chem. 2012; 287(24):20221-30.

PMID: 22528499 PMC: 3370204. DOI: 10.1074/jbc.M111.334334.

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