Three-dimensional Structure of the Elastase of Pseudomonas Aeruginosa at 1.5-A Resolution

Overview

Journal J Biol Chem

Specialty Biochemistry

Date 1991 Feb 15

PMID 1899664

Citations 61

Authors

M M Thayer

K M Flaherty

D B McKay

Affiliations

Soon will be listed here.

Abstract

Pseudomonas aeruginosa elastase (PAE) is a zinc metalloprotease with 301 amino acids. We have crystallized and solved the three-dimensional structure of PAE, using data to 1.5-A resolution, and have refined the native molecular structure to R = 0.188. The overall tertiary structure of the PAE molecule is similar to that of thermolysin, with which it shares 28% amino acid sequence identity. Nearly all of the active site residues that might potentially interact with substrates are identical in the two proteins. However, the active site cleft is significantly more "open" in PAE than in thermolysin.

Citing Articles

Potential of Marine Bacterial Metalloprotease A69 in the Preparation of Peanut Peptides with Angiotensin-Converting Enzyme (ACE)-Inhibitory and Antioxidant Properties.

Cao W, Liu R, Zhao W, Li J, Wang Y, Yuan X Mar Drugs. 2024; 22(7).

PMID: 39057414 PMC: 11277839. DOI: 10.3390/md22070305.

and the Complement System: A Review of the Evasion Strategies.

Gonzalez-Alsina A, Mateu-Borras M, Domenech-Sanchez A, Alberti S Microorganisms. 2023; 11(3).

PMID: 36985237 PMC: 10056308. DOI: 10.3390/microorganisms11030664.

Extract as a Promising Antimicrobial and Antivirulence Therapy.

Ghaly M, Albalawi M, Bendary M, Shahin A, Shaheen M, Abu Eleneen A Antibiotics (Basel). 2023; 12(3).

PMID: 36978330 PMC: 10044421. DOI: 10.3390/antibiotics12030464.

Chemical Optimization of Selective LasB Elastase Inhibitors and Their Impact on LasB-Mediated Activation of IL-1β in Cellular and Animal Infection Models.

Everett M, Davies D, Leiris S, Sprynski N, Llanos A, Castandet J ACS Infect Dis. 2023; 9(2):270-282.

PMID: 36669138 PMC: 9926489. DOI: 10.1021/acsinfecdis.2c00418.

Direct Bactericidal Comparison of Metal Nanoparticles and Their Salts against . Culture by TEM and FT-IR Spectroscopy.

Saraeva I, Tolordava E, Yushina Y, Sozaev I, Sokolova V, Khmelnitskiy R Nanomaterials (Basel). 2022; 12(21).

PMID: 36364634 PMC: 9657403. DOI: 10.3390/nano12213857.