The Effect of Phorbol Ester Treatment on Migration of C3H 10T1/2 and BT5C Glioma Cells: Possible Application to Carcinogenesis

Overview

Journal J Cancer Res Clin Oncol

Specialty Oncology

Date 1994 Jan 1

PMID 8263011

Citations 1

Authors

P Janik

B Szaniawska

D Kowalczyk

J Miloszewska

Affiliations

Soon will be listed here.

Abstract

Treatment with the tumor-promoting phorbol ester 12-O-tetradecanoylphorbol 13-acetate (TPA) and the second-stage promoter 12-O-retinoylphorbol 13-acetate induced down-regulation of protein kinase C (PKC) and enhanced the migration of C3H 10T1/2 cells. In the case of BT5C glioma cells the same negative correlation was observed only after treatment with TPA. The negative control 4 alpha-phorbol affected neither PKC activity nor the migratory ability of both cell lines.

Citing Articles

Gelsolin suppresses tumorigenicity through inhibiting PKC activation in a human lung cancer cell line, PC10.

Sagawa N, Fujita H, Banno Y, Nozawa Y, Katoh H, Kuzumaki N Br J Cancer. 2003; 88(4):606-12.

PMID: 12592377 PMC: 2377162. DOI: 10.1038/sj.bjc.6600739.

References

Dugina V, Svitkina T, Vasiliev J, Gelfand I . Special type of morphological reorganization induced by phorbol ester: reversible partition of cell into motile and stable domains. Proc Natl Acad Sci U S A. 1987; 84(12):4122-5. PMC: 305035. DOI: 10.1073/pnas.84.12.4122. View

Kennedy A, Little J . Investigation of the mechanism for enhancement of radiation transformation in vitro by 12-O-tetradecanoylphorbol-13-acetate. Carcinogenesis. 1980; 1(12):1039-47. DOI: 10.1093/carcin/1.12.1039. View

Rodriguez-Pena A, Rozengurt E . Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cells. Biochem Biophys Res Commun. 1984; 120(3):1053-9. DOI: 10.1016/s0006-291x(84)80213-2. View

Kraft A, Anderson W . Phorbol esters increase the amount of Ca2+, phospholipid-dependent protein kinase associated with plasma membrane. Nature. 1983; 301(5901):621-3. DOI: 10.1038/301621a0. View

Rosen E, Jaken S, Carley W, Luckett P, Setter E, Bhargava M . Regulation of motility in bovine brain endothelial cells. J Cell Physiol. 1991; 146(2):325-35. DOI: 10.1002/jcp.1041460218. View

Gschwendt M, Horn F, Kittstein W, Furstenberger G, Marks F . Soluble phorbol ester binding sites and phospholipid- and calcium-dependent protein kinase activity in cytosol of chick oviduct. FEBS Lett. 1983; 162(1):147-50. DOI: 10.1016/0014-5793(83)81067-9. View

Chida K, Hashiba H, Sasaki K, Kuroki T . Activation of protein kinase C and specific phosphorylation of a Mr 90,000 membrane protein of promotable BALB/3T3 and C3H/10T1/2 cells by tumor promoters. Cancer Res. 1986; 46(3):1055-62. View

Reznikoff C, Bertram J, Brankow D, HEIDELBERGER C . Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 1973; 33(12):3239-49. View

Szaniawska B, Bjerkvig R, Laerum O, Undheim K . Growth arrest and polyploidization induced by metahalone microtubule inhibitors on rat glioma cells in culture. Anticancer Res. 1986; 6(1):71-7. View

10.

Droms K, Malkinson A . Phorbol ester-induced tumor promotion by downregulation of protein kinase C. Mol Carcinog. 1991; 4(1):1-2. DOI: 10.1002/mc.2940040102. View

11.

Castagna M, Takai Y, Kaibuchi K, Sano K, Kikkawa U, NISHIZUKA Y . Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982; 257(13):7847-51. View

12.

Schwartz G, Redwood S, Ohnuma T, HOLLAND J, Droller M, Liu B . Inhibition of invasion of invasive human bladder carcinoma cells by protein kinase C inhibitor staurosporine. J Natl Cancer Inst. 1990; 82(22):1753-6. DOI: 10.1093/jnci/82.22.1753. View

13.

Hansen L, Monteiro-Riviere N, Smart R . Differential down-regulation of epidermal protein kinase C by 12-O-tetradecanoylphorbol-13-acetate and diacylglycerol: association with epidermal hyperplasia and tumor promotion. Cancer Res. 1990; 50(18):5740-5. View

14.

Mordan L, Bergin L, Budnick J, Meegan R, Bertram J . Isolation of methylcholanthrene-"initiated" C3H/10T1/2 cells by inhibiting neoplastic progression with retinyl acetate. Carcinogenesis. 1982; 3(3):279-85. DOI: 10.1093/carcin/3.3.279. View

15.

Janik P, Szaniawska B, Miloszewska J, Pietruszewska E, Kowalczyk D . The role of protein kinase C in migration of rat glioma cells from spheroid cultures. Cancer Lett. 1992; 63(2):167-70. DOI: 10.1016/0304-3835(92)90067-6. View

16.

NISHIZUKA Y . The role of protein kinase C in cell surface signal transduction and tumour promotion. Nature. 1984; 308(5961):693-8. DOI: 10.1038/308693a0. View

17.

Miloszewska J, Trawicki W, Janik P, Moraczewski J, Przybyszewska M, Szaniawska B . Protein kinase C translocation in relation to proliferative state of C3H 10T1/2 cells. FEBS Lett. 1986; 206(2):283-6. DOI: 10.1016/0014-5793(86)80997-8. View

18.

Schwartz G, Jiang J, Kelsen D, Albino A . Protein kinase C: a novel target for inhibiting gastric cancer cell invasion. J Natl Cancer Inst. 1993; 85(5):402-7. DOI: 10.1093/jnci/85.5.402. View

19.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

20.

Yuhas J, Li A, Martinez A, LADMAN A . A simplified method for production and growth of multicellular tumor spheroids. Cancer Res. 1977; 37(10):3639-43. View