Protein Kinase C Translocation in Relation to Proliferative State of C3H 10T1/2 Cells

Overview

Journal FEBS Lett

Specialty Biochemistry

Date 1986 Oct 6

PMID 3758354

Citations 7

Authors

J Miloszewska

W Trawicki

P Janik

J Moraczewski

M Przybyszewska

B Szaniawska

Affiliations

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Abstract

Protein kinase C (PKC) activity of cytosol and membrane fractions of 10T1/2 cells was studied. In cytosol of fast growing cells PKC activity was found in material eluted with 0.150 M NaCl whereas in membrane fractions activity was eluted with 0.065 and 0.150 M NaCl. In the membrane fraction of confluent cells, in contrast to cytosol, very low PKC activity was observed. The translocation pattern of PKC activity eluted with 0.065 M NaCl may be associated with proliferation.

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