Enhanced Replication of Porcine Reproductive and Respiratory Syndrome (PRRS) Virus in a Homogeneous Subpopulation of MA-104 Cell Line

Overview

Journal Arch Virol

Specialty Microbiology

Date 1993 Jan 1

PMID 8257302

Citations 250

Authors

H S Kim

J Kwang

I J Yoon

H S Joo

M L Frey

Affiliations

Soon will be listed here.

Abstract

Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 10(8.5), 10(3.5), and 10(2.5) tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.

Citing Articles

Establishment of an Immortalized Porcine Alveolar Macrophage Cell Line That Supports Efficient Replication of Porcine Reproductive and Respiratory Syndrome Viruses.

Diep N, Hayakawa-Sugaya Y, Ishikawa S, Kawaguchi H, Suda Y, Esaki M Pathogens. 2025; 13(12.

PMID: 39770286 PMC: 11678208. DOI: 10.3390/pathogens13121026.

The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating.

Yang K, Dong J, Li J, Zhou R, Jia X, Sun Z J Virol. 2024; 99(1):e0121824.

PMID: 39651859 PMC: 11784455. DOI: 10.1128/jvi.01218-24.

Porcine reproductive and respiratory syndrome virus degrades TANK-binding kinase 1 via chaperon-mediated autophagy to suppress type I interferon production and facilitate viral proliferation.

Zhao S, Qian Q, Wang Y, Qiao S, Li R Vet Res. 2024; 55(1):151.

PMID: 39543624 PMC: 11566183. DOI: 10.1186/s13567-024-01392-w.

Linear epitopes of PRRSV-1 envelope proteins ectodomains are not correlated with broad neutralization.

Castillo-Perez J, Martinez-Lobo F, Frometa R, Castro J, Simarro I, Prieto C Porcine Health Manag. 2024; 10(1):44.

PMID: 39434120 PMC: 11492654. DOI: 10.1186/s40813-024-00393-7.

A Comprehensive Review on Porcine Reproductive and Respiratory Syndrome Virus with Emphasis on Immunity.

Fiers J, Cay A, Maes D, Tignon M Vaccines (Basel). 2024; 12(8).

PMID: 39204065 PMC: 11359659. DOI: 10.3390/vaccines12080942.

References

Christianson W, Collins J, Benfield D, Harris L, Gorcyca D, Chladek D . Experimental reproduction of swine infertility and respiratory syndrome in pregnant sows. Am J Vet Res. 1992; 53(4):485-8. View

Benfield D, Nelson E, Collins J, Harris L, Goyal S, Robison D . Characterization of swine infertility and respiratory syndrome (SIRS) virus (isolate ATCC VR-2332). J Vet Diagn Invest. 1992; 4(2):127-33. DOI: 10.1177/104063879200400202. View

Meulenberg J, Hulst M, de Meijer E, Moonen P, den Besten A, de Kluyver E . Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology. 1993; 192(1):62-72. PMC: 7173055. DOI: 10.1006/viro.1993.1008. View

Morrison R, Collins J, Harris L, Christianson W, Benfield D, Chladek D . Serologic evidence incriminating a recently isolated virus (ATCC VR-2332) as the cause of swine infertility and respiratory syndrome (SIRS). J Vet Diagn Invest. 1992; 4(2):186-8. DOI: 10.1177/104063879200400212. View

Yoon I, Joo H, Christianson W, Kim H, Collins J, Morrison R . An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J Vet Diagn Invest. 1992; 4(2):144-7. DOI: 10.1177/104063879200400205. View

Collins J, Benfield D, Christianson W, Harris L, Hennings J, Shaw D . Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest. 1992; 4(2):117-26. DOI: 10.1177/104063879200400201. View

Plagemann P, Moennig V . Lactate dehydrogenase-elevating virus, equine arteritis virus, and simian hemorrhagic fever virus: a new group of positive-strand RNA viruses. Adv Virus Res. 1992; 41:99-192. PMC: 7131515. DOI: 10.1016/s0065-3527(08)60036-6. View

Wensvoort G, Terpstra C, Pol J, ter Laak E, Bloemraad M, de Kluyver E . Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q. 1991; 13(3):121-30. DOI: 10.1080/01652176.1991.9694296. View

Conzelmann K, Visser N, van Woensel P, Thiel H . Molecular characterization of porcine reproductive and respiratory syndrome virus, a member of the arterivirus group. Virology. 1993; 193(1):329-39. PMC: 7131490. DOI: 10.1006/viro.1993.1129. View

10.

Terpstra C, Wensvoort G, Pol J . Experimental reproduction of porcine epidemic abortion and respiratory syndrome (mystery swine disease) by infection with Lelystad virus: Koch's postulates fulfilled. Vet Q. 1991; 13(3):131-6. DOI: 10.1080/01652176.1991.9694297. View

11.

. 'Blue ear' disease of pigs. Vet Rec. 1991; 128(24):574. DOI: 10.1136/vr.128.24.574. View

12.

Yoon I, Joo H, Christianson W, Kim H, Collins J, Carlson J . Isolation of a cytopathic virus from weak pigs on farms with a history of swine infertility and respiratory syndrome. J Vet Diagn Invest. 1992; 4(2):139-43. DOI: 10.1177/104063879200400204. View

13.

Wensvoort G, de Kluyver E, Luijtze E, den Besten A, Harris L, Collins J . Antigenic comparison of Lelystad virus and swine infertility and respiratory syndrome (SIRS) virus. J Vet Diagn Invest. 1992; 4(2):134-8. DOI: 10.1177/104063879200400203. View