Minor Envelope Proteins from GP2a to GP4 Contribute to the Spread Pattern and Yield of Type 2 PRRSV in MARC-145 Cells

Overview

Journal Front Cell Infect Microbiol

Specialties Cell Biology
Infectious Diseases
Microbiology

Date 2024 Apr 9

PMID 38590440

Authors

Yuan-Zhe Bai

Yue Sun

Yong-Gang Liu

Hong-Liang Zhang

Tong-Qing An

Qian Wang

Zhi-Jun Tian

Xinyuan Qiao

Xue-Hui Cai

Yan-Dong Tang

Affiliations

Soon will be listed here.

Abstract

In China, porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are widely used. These vaccines, which contain inactivated and live attenuated vaccines (LAVs), are produced by MARC-145 cells derived from the monkey kidney cell line. However, some PRRSV strains in MARC-145 cells have a low yield. Here, we used two type 2 PRRSV strains (CH-1R and HuN4) to identify the genes responsible for virus yield in MARC-145 cells. Our findings indicate that the two viruses have different spread patterns, which ultimately determine their yield. By replacing the viral envelope genes with a reverse genetics system, we discovered that the minor envelope proteins, from GP2a to GP4, play a crucial role in determining the spread pattern and yield of type 2 PRRSV in MARC-145 cells. The cell-free transmission pattern of type 2 PRRSV appears to be more efficient than the cell-to-cell transmission pattern. Overall, these findings suggest that GP2a to GP4 contributes to the spread pattern and yield of type 2 PRRSV.

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References

Wang T, Liu Y, Li L, Wang G, Wang H, Zhang H . Porcine alveolar macrophage CD163 abundance is a pivotal switch for porcine reproductive and respiratory syndrome virus infection. Oncotarget. 2018; 9(15):12174-12185. PMC: 5844737. DOI: 10.18632/oncotarget.24040. View

Yang Y, Liu J, Wang T, Chen M, Wang G, Yang Y . Aminopeptidase N Is an Entry Co-factor Triggering Porcine Deltacoronavirus Entry via an Endocytotic Pathway. J Virol. 2021; 95(21):e0094421. PMC: 8513460. DOI: 10.1128/JVI.00944-21. View

Wang T, Meng F, Sang G, Zhang H, Tian Z, Zheng H . A novel viral vaccine platform based on engineered transfer RNA. Emerg Microbes Infect. 2022; 12(1):2157339. PMC: 9769134. DOI: 10.1080/22221751.2022.2157339. View

Zhang Q, Yoo D . PRRS virus receptors and their role for pathogenesis. Vet Microbiol. 2015; 177(3-4):229-41. DOI: 10.1016/j.vetmic.2015.04.002. View

Zhang H, Li Y, Sun J, Zhang Y, Wang T, Sun M . Evaluating angiotensin-converting enzyme 2-mediated SARS-CoV-2 entry across species. J Biol Chem. 2021; 296:100435. PMC: 7892319. DOI: 10.1016/j.jbc.2021.100435. View

Yim-Im W, Huang H, Park J, Wang C, Calzada G, Gauger P . Comparison of ZMAC and MARC-145 Cell Lines for Improving Porcine Reproductive and Respiratory Syndrome Virus Isolation from Clinical Samples. J Clin Microbiol. 2020; 59(3). PMC: 8106717. DOI: 10.1128/JCM.01757-20. View

Duan X, Nauwynck H, Pensaert M . Effects of origin and state of differentiation and activation of monocytes/macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV). Arch Virol. 1997; 142(12):2483-97. PMC: 7086874. DOI: 10.1007/s007050050256. View

Gorbalenya A, Enjuanes L, Ziebuhr J, Snijder E . Nidovirales: evolving the largest RNA virus genome. Virus Res. 2006; 117(1):17-37. PMC: 7114179. DOI: 10.1016/j.virusres.2006.01.017. View

Cafruny W, Duman R, Wong G, Said S, Ward-Demo P, Rowland R . Porcine reproductive and respiratory syndrome virus (PRRSV) infection spreads by cell-to-cell transfer in cultured MARC-145 cells, is dependent on an intact cytoskeleton, and is suppressed by drug-targeting of cell permissiveness to virus infection. Virol J. 2006; 3:90. PMC: 1635561. DOI: 10.1186/1743-422X-3-90. View

10.

Guo R, Katz B, Tomich J, Gallagher T, Fang Y . Porcine Reproductive and Respiratory Syndrome Virus Utilizes Nanotubes for Intercellular Spread. J Virol. 2016; 90(10):5163-5175. PMC: 4859731. DOI: 10.1128/JVI.00036-16. View

11.

Nan Y, Wu C, Gu G, Sun W, Zhang Y, Zhou E . Improved Vaccine against PRRSV: Current Progress and Future Perspective. Front Microbiol. 2017; 8:1635. PMC: 5581347. DOI: 10.3389/fmicb.2017.01635. View

12.

Tang Y, Fang Q, Liu J, Wang T, Wang Y, Tao Y . Open reading frames 1a and 1b of the porcine reproductive and respiratory syndrome virus (PRRSV) collaboratively initiate viral minus-strand RNA synthesis. Biochem Biophys Res Commun. 2016; 477(4):927-931. DOI: 10.1016/j.bbrc.2016.06.161. View

13.

Wensvoort G, Terpstra C, Pol J, ter Laak E, Bloemraad M, de Kluyver E . Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q. 1991; 13(3):121-30. DOI: 10.1080/01652176.1991.9694296. View

14.

Wang T, Fang Q, Cong F, Liu Y, Wang H, Zhang H . The Nsp12-coding region of type 2 PRRSV is required for viral subgenomic mRNA synthesis. Emerg Microbes Infect. 2019; 8(1):1501-1510. PMC: 6818116. DOI: 10.1080/22221751.2019.1679010. View

15.

Tong G, Zhou Y, Hao X, Tian Z, An T, Qiu H . Highly pathogenic porcine reproductive and respiratory syndrome, China. Emerg Infect Dis. 2008; 13(9):1434-6. PMC: 2857295. DOI: 10.3201/eid1309.070399. View

16.

Meulenberg J . PRRSV, the virus. Vet Res. 2000; 31(1):11-21. DOI: 10.1051/vetres:2000103. View

17.

Wang T, Sang G, Wang Q, Leng C, Tian Z, Peng J . Generation of Premature Termination Codon (PTC)-Harboring Pseudorabies Virus (PRV) via Genetic Code Expansion Technology. Viruses. 2022; 14(3). PMC: 8950157. DOI: 10.3390/v14030572. View

18.

Zhong P, Agosto L, Munro J, Mothes W . Cell-to-cell transmission of viruses. Curr Opin Virol. 2012; 3(1):44-50. PMC: 3587356. DOI: 10.1016/j.coviro.2012.11.004. View

19.

Kim H, Kwang J, Yoon I, Joo H, Frey M . Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line. Arch Virol. 1993; 133(3-4):477-83. DOI: 10.1007/BF01313785. View

20.

Tang Y, Yu C, Cai X . Novel technologies are turning a dream into reality: conditionally replicating viruses as vaccines. Trends Microbiol. 2023; 32(3):292-301. DOI: 10.1016/j.tim.2023.09.002. View