Evidence of Two Levels of Control of P1 OriR and Host OriC Replication Origins by DNA Adenine Methylation

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1993 Dec 1

PMID 8253669

Citations 13

Authors

A Abeles

T Brendler

S Austin

Affiliations

Soon will be listed here.

Abstract

A mutant mini-P1 plasmid with increased copy number can be established in Dam- strains of Escherichia coli, where mini-P1 plasmid replication is normally blocked. Comparison of this plasmid and a plasmid driven by the host oriC replication origin showed that both origins are subject to control by methylation at two different levels. First, both origins appear to be subject to negative regulation acting at the level of hemimethylation. This probably involves the sequestration of the hemimethylated DNA produced by replication, as has been previously described for oriC. Second, both origins show a positive requirement for adenine methylation for efficient function in vivo. This conclusion is supported by the behavior of the P1 origin in an improved in vitro replication system. In vitro, where sequestration of hemimethylated DNA is not expected to occur, the hemimethylated P1 origin DNA was fully functional as a template. However, the activity of fully unmethylated DNA was severely restricted in comparison with that of either of the methylated forms. This in vitro uncoupling of the two effects of origin methylation suggests that two separate mechanisms are involved.

Citing Articles

An Adversarial DNA N-Methyladenine-Sensor Network Preserves Polycomb Silencing.

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Adenine methylation in eukaryotes: Apprehending the complex evolutionary history and functional potential of an epigenetic modification.

Iyer L, Zhang D, Aravind L Bioessays. 2015; 38(1):27-40.

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DNA Methylation.

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Computational identification of novel biochemical systems involved in oxidation, glycosylation and other complex modifications of bases in DNA.

Iyer L, Zhang D, Burroughs A, Aravind L Nucleic Acids Res. 2013; 41(16):7635-55.

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Replication of Vibrio cholerae chromosome I in Escherichia coli: dependence on dam methylation.

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