The Hemimethylated Replication Origin of Escherichia Coli Can Be Initiated in Vitro

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1991 Jul 1

PMID 2066345

Citations 7

Authors

E Boye

Affiliations

Soon will be listed here.

Abstract

Unmethylated, fully methylated, and hemimethylated oriC-containing plasmids were assayed as substrates for DNA replication in vitro by using a system reconstituted with pure proteins. In contrast to the in vivo situation, all three substrates were initiated efficiently; the fully methylated plasmid was about twice as active as the other two.

Citing Articles

Excess SeqA prolongs sequestration of oriC and delays nucleoid segregation and cell division.

Bach T, Krekling M, Skarstad K EMBO J. 2003; 22(2):315-23.

PMID: 12514137 PMC: 140095. DOI: 10.1093/emboj/cdg020.

Limiting DNA replication to once and only once.

Boye E, Lobner-Olesen A, Skarstad K EMBO Rep. 2001; 1(6):479-83.

PMID: 11263490 PMC: 1083788. DOI: 10.1093/embo-reports/kvd116.

The eclipse period of Escherichia coli.

von Freiesleben U, Krekling M, Hansen F, Lobner-Olesen A EMBO J. 2000; 19(22):6240-8.

PMID: 11080169 PMC: 305828. DOI: 10.1093/emboj/19.22.6240.

Evidence of two levels of control of P1 oriR and host oriC replication origins by DNA adenine methylation.

Abeles A, Brendler T, Austin S J Bacteriol. 1993; 175(24):7801-7.

PMID: 8253669 PMC: 206955. DOI: 10.1128/jb.175.24.7801-7807.1993.

The initiation cascade for chromosome replication in wild-type and Dam methyltransferase deficient Escherichia coli cells.

Lobner-Olesen A, Hansen F, Rasmussen K, Martin B, KUEMPEL P EMBO J. 1994; 13(8):1856-62.

PMID: 8168484 PMC: 395025. DOI: 10.1002/j.1460-2075.1994.tb06454.x.

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