Effects of Cold Environment on Mitochondrial Genome Expression in the Rat: Evidence for a Tissue-specific Increase in the Liver, Independent of Changes in Mitochondrial Gene Abundance

Overview

Journal Biochem J

Specialty Biochemistry

Date 1993 Nov 15

PMID 8250848

Citations 6

Authors

I Martin

O Vinas

T Mampel

R Iglesias

F Villarroya

Affiliations

Soon will be listed here.

Abstract

The abundance of the mitochondrially encoded mRNA for subunit II of cytochrome c oxidase (COII mRNA) increases in the liver of rats exposed to environmental cold stress (4 degrees C ambient temperature). Only transient increases or no changes in COII mRNA levels were observed in brown fat and soleus muscle respectively. The time course of the liver COII mRNA increase was compared with the effects of cold stress on mitochondrial 16S ribosomal RNA expression and indicated that cold induces a rapid (few hours) increase in the liver mitochondrial mRNA levels and high levels of both messenger and ribosomal RNA mitochondrial transcripts are present after a few days of cold exposure. No changes in mitochondrial DNA abundance relative to total cellular DNA were observed in the liver of rats at any time during cold stress. It is concluded that mitochondrial genome expression is specifically increased in the liver of cold-exposed rats through different mechanisms, independent of changes in mitochondrial genome abundance.

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References

Williams R . Mitochondrial gene expression in mammalian striated muscle. Evidence that variation in gene dosage is the major regulatory event. J Biol Chem. 1986; 261(26):12390-4. View

Christianson T, Clayton D . A tridecamer DNA sequence supports human mitochondrial RNA 3'-end formation in vitro. Mol Cell Biol. 1988; 8(10):4502-9. PMC: 365525. DOI: 10.1128/mcb.8.10.4502-4509.1988. View

Cantatore P, Saccone C . Organization, structure, and evolution of mammalian mitochondrial genes. Int Rev Cytol. 1987; 108:149-208. DOI: 10.1016/s0074-7696(08)61438-2. View

Lomedico P, Saunders G . Preparation of pancreatic mRNA: cell-free translation of an insulin-immunoreactive polypeptide. Nucleic Acids Res. 1976; 3(2):381-91. PMC: 342908. DOI: 10.1093/nar/3.2.381. View

Behrens W, Himms-Hagen J . Alteration in skeletal muscle mitochondria of cold-acclimated rats: association with enhanced metabolic response to noradrenaline. J Bioenerg Biomembr. 1977; 9(1):41-63. DOI: 10.1007/BF00745042. View

Castora F, Arnheim N, Simpson M . Mitochondrial DNA polymorphism: evidence that variants detected by restriction enzymes differ in nucleotide sequence rather than in methylation. Proc Natl Acad Sci U S A. 1980; 77(11):6415-9. PMC: 350295. DOI: 10.1073/pnas.77.11.6415. View

Nudel U, Zakut R, Shani M, Neuman S, Levy Z, Yaffe D . The nucleotide sequence of the rat cytoplasmic beta-actin gene. Nucleic Acids Res. 1983; 11(6):1759-71. PMC: 325834. DOI: 10.1093/nar/11.6.1759. View

Attardi G . Animal mitochondrial DNA: an extreme example of genetic economy. Int Rev Cytol. 1985; 93:93-145. DOI: 10.1016/s0074-7696(08)61373-x. View

Glaichenhaus N, Leopold P, Cuzin F . Increased levels of mitochondrial gene expression in rat fibroblast cells immortalized or transformed by viral and cellular oncogenes. EMBO J. 1986; 5(6):1261-5. PMC: 1166936. DOI: 10.1002/j.1460-2075.1986.tb04355.x. View

10.

Goglia F, Liverini G, Lanni A, Barletta A . Mitochondrial DNA, RNA and protein synthesis in normal, hypothyroid and mildly hyperthyroid rat liver during cold exposure. Mol Cell Endocrinol. 1988; 55(2-3):141-7. DOI: 10.1016/0303-7207(88)90128-1. View

11.

Van Itallie C, Dannies P . Estrogen induces accumulation of the mitochondrial ribonucleic acid for subunit II of cytochrome oxidase in pituitary tumor cells. Mol Endocrinol. 1988; 2(4):332-7. DOI: 10.1210/mend-2-4-332. View

12.

Kadowaki T, Kitagawa Y . Enhanced transcription of mitochondrial genes after growth stimulation and glucocorticoid treatment of Reuber hepatoma H-35. FEBS Lett. 1988; 233(1):51-6. DOI: 10.1016/0014-5793(88)81354-1. View

13.

Harley C . Hybridization of oligo(dT) to RNA on nitrocellulose. Gene Anal Tech. 1987; 4(2):17-22. DOI: 10.1016/0735-0651(87)90013-6. View

14.

Mutvei A, KUZELA S, Nelson B . Control of mitochondrial transcription by thyroid hormone. Eur J Biochem. 1989; 180(1):235-40. DOI: 10.1111/j.1432-1033.1989.tb14638.x. View

15.

Martin I, Giralt M, Vinas O, Iglesias R, Mampel T, Villarroya F . Adaptative decrease in expression of the mRNA for uncoupling protein and subunit II of cytochrome c oxidase in rat brown adipose tissue during pregnancy and lactation. Biochem J. 1989; 263(3):965-8. PMC: 1133525. DOI: 10.1042/bj2630965. View

16.

Lomax M, Grossman L . Tissue-specific genes for respiratory proteins. Trends Biochem Sci. 1989; 14(12):501-3. DOI: 10.1016/0968-0004(89)90185-0. View

17.

Van Itallie C . Thyroid hormone and dexamethasone increase the levels of a messenger ribonucleic acid for a mitochondrially encoded subunit but not for a nuclear-encoded subunit of cytochrome c oxidase. Endocrinology. 1990; 127(1):55-62. DOI: 10.1210/endo-127-1-55. View

18.

Nelson B . Thyroid hormone regulation of mitochondrial function. Comments on the mechanism of signal transduction. Biochim Biophys Acta. 1990; 1018(2-3):275-7. DOI: 10.1016/0005-2728(90)90266-7. View

19.

Clayton D . Nuclear gadgets in mitochondrial DNA replication and transcription. Trends Biochem Sci. 1991; 16(3):107-11. DOI: 10.1016/0968-0004(91)90043-u. View

20.

Iossa S, Liverini G, Barletta A . Effect of thyroid state and cold exposure on rat liver mitochondrial protein mass and function. J Endocrinol. 1991; 131(1):67-73. DOI: 10.1677/joe.0.1310067. View