Amino Acid Substitutions in the Floor of the Putative Antigen-binding Site of H-2T22 Affect Recognition by a Gamma Delta T-cell Receptor

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1993 Dec 1

PMID 8248260

Citations 5

Authors

S Moriwaki

B S Korn

Y Ichikawa

L Van Kaer

S Tonegawa

Affiliations

Soon will be listed here.

Abstract

We have previously identified a self-reactive gamma delta T-cell clone (KN6) specific for the H-2T region gene product T22b. Now we have investigated by an in vitro mutagenesis analysis of the T22b gene the possibility that the interaction between the KN6 gamma delta T-cell receptor and T22b involves a peptide. The results demonstrate that mutations at the floor of the putative antigen-binding groove of T22b affect recognition by the gamma delta T-cell receptor. Furthermore, we have shown that KN6 cells react with cells that are deficient in the class I peptide transporter TAP1/TAP2. These results suggest that peptide is involved in the interaction of the KN6 T-cell receptor with T22 and that loading of T22 with the putative peptide is TAP1/TAP2-independent.

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References

Shapiro M, Kasper D, Zaleznik D, Spriggs S, Onderdonk A, Finberg R . Cellular control of abscess formation: role of T cells in the regulation of abscesses formed in response to Bacteroides fragilis. J Immunol. 1986; 137(1):341-6. View

Shepherd J, Schumacher T, Ashton-Rickardt P, IMAEDA S, Ploegh H, Janeway Jr C . TAP1-dependent peptide translocation in vitro is ATP dependent and peptide selective. Cell. 1993; 74(3):577-84. DOI: 10.1016/0092-8674(93)80058-m. View

Maeda K, Nakanishi N, Rogers B, Haser W, Shitara K, Yoshida H . Expression of the T-cell receptor gamma-chain gene products on the surface of peripheral T cells and T-cell blasts generated by allogeneic mixed lymphocyte reaction. Proc Natl Acad Sci U S A. 1987; 84(18):6536-40. PMC: 299112. DOI: 10.1073/pnas.84.18.6536. View

Bjorkman P, Saper M, Samraoui B, Bennett W, Strominger J, Wiley D . Structure of the human class I histocompatibility antigen, HLA-A2. Nature. 1987; 329(6139):506-12. DOI: 10.1038/329506a0. View

Bluestone J, Cron R, Cotterman M, Houlden B, Matis L . Structure and specificity of T cell receptor gamma/delta on major histocompatibility complex antigen-specific CD3+, CD4-, CD8- T lymphocytes. J Exp Med. 1988; 168(5):1899-916. PMC: 2189096. DOI: 10.1084/jem.168.5.1899. View

Strominger J . The gamma delta T cell receptor and class Ib MHC-related proteins: enigmatic molecules of immune recognition. Cell. 1989; 57(6):895-8. DOI: 10.1016/0092-8674(89)90326-7. View

Ciccone E, Viale O, Pende D, Malnati M, Battista Ferrara G, Barocci S . Specificity of human T lymphocytes expressing a gamma/delta T cell antigen receptor. Recognition of a polymorphic determinant of HLA class I molecules by a gamma/delta clone. Eur J Immunol. 1989; 19(7):1267-71. DOI: 10.1002/eji.1830190718. View

Spits H, Paliard X, De Vries J . Antigen-specific, but not natural killer, activity of T cell receptor-gamma delta cytotoxic T lymphocyte clones involves secretion of N alpha-benzyloxycarbonyl-L-lysine thiobenzyl ester serine esterase and influx of Ca2+ ions. J Immunol. 1989; 143(5):1506-11. View

Bonneville M, Ito K, Krecko E, Itohara S, Kappes D, Ishida I . Recognition of a self major histocompatibility complex TL region product by gamma delta T-cell receptors. Proc Natl Acad Sci U S A. 1989; 86(15):5928-32. PMC: 297744. DOI: 10.1073/pnas.86.15.5928. View

10.

Vidovic D, Roglic M, McKune K, Guerder S, MacKay C, Dembic Z . Qa-1 restricted recognition of foreign antigen by a gamma delta T-cell hybridoma. Nature. 1989; 340(6235):646-50. DOI: 10.1038/340646a0. View

11.

Matis L, Fry A, Cron R, Cotterman M, Dick R, Bluestone J . Structure and specificity of a class II MHC alloreactive gamma delta T cell receptor heterodimer. Science. 1989; 245(4919):746-9. DOI: 10.1126/science.2528206. View

12.

Janeway Jr C, Jones B, Hayday A . Specificity and function of T cells bearing gamma delta receptors. Immunol Today. 1988; 9(3):73-6. DOI: 10.1016/0167-5699(88)91267-4. View

13.

Hemsley A, Arnheim N, Toney M, Cortopassi G, Galas D . A simple method for site-directed mutagenesis using the polymerase chain reaction. Nucleic Acids Res. 1989; 17(16):6545-51. PMC: 318348. DOI: 10.1093/nar/17.16.6545. View

14.

Porcelli S, Brenner M, Greenstein J, Balk S, Terhorst C, Bleicher P . Recognition of cluster of differentiation 1 antigens by human CD4-CD8-cytolytic T lymphocytes. Nature. 1989; 341(6241):447-50. DOI: 10.1038/341447a0. View

15.

Ishida I, Verbeek S, Bonneville M, Itohara S, Berns A, Tonegawa S . T-cell receptor gamma delta and gamma transgenic mice suggest a role of a gamma gene silencer in the generation of alpha beta T cells. Proc Natl Acad Sci U S A. 1990; 87(8):3067-71. PMC: 53835. DOI: 10.1073/pnas.87.8.3067. View

16.

Spits H, Paliard X, Engelhard V, De Vries J . Cytotoxic activity and lymphokine production of T cell receptor (TCR)-alpha beta+ and TCR-gamma delta+ cytotoxic T lymphocyte (CTL) clones recognizing HLA-A2 and HLA-A2 mutants. Recognition of TCR-gamma delta+ CTL clones is affected by mutations at.... J Immunol. 1990; 144(11):4156-62. View

17.

Lam V, Demars R, Chen B, Hank J, Kovats S, Fisch P . Human T cell receptor-gamma delta-expressing T-cell lines recognize MHC-controlled elements on autologous EBV-LCL that are not HLA-A, -B, -C, -DR, -DQ, or -DP. J Immunol. 1990; 145(1):36-45. View

18.

Born W, Hall L, Dallas A, Boymel J, Shinnick T, Young D . Recognition of a peptide antigen by heat shock--reactive gamma delta T lymphocytes. Science. 1990; 249(4964):67-9. DOI: 10.1126/science.1695022. View

19.

Tonegawa S, Berns A, Bonneville M, Farr A, Ishida I, Ito K . Diversity, development, ligands, and probable functions of gamma delta T cells. Cold Spring Harb Symp Quant Biol. 1989; 54 Pt 1:31-44. DOI: 10.1101/sqb.1989.054.01.005. View

20.

Houlden B, Matis L, Cron R, Widacki S, Brown G, Pampeno C . A TCR gamma delta cell recognizing a novel TL-encoded gene product. Cold Spring Harb Symp Quant Biol. 1989; 54 Pt 1:45-55. DOI: 10.1101/sqb.1989.054.01.006. View