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Basic Electrical Properties of Tight Epithelia Determined with a Simple Method

Overview
Journal Pflugers Arch
Specialty Physiology
Date 1976 Jun 29
PMID 822395
Citations 12
Authors
D Erlij
Affiliations
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Abstract

A simple method for the routine mounting of epithelia which greatly reduces or eliminates faulty sealing at the edge is described. The values of potential and resistance observed in the frog skin and toad urinary bladder are comparable to those found with more complicated methods designed to minimize edge damage. In agreement with observations in other epithelia, a direct relationship between short circuit and conductance is observed for frog skin and toad urinary bladder, implying that the relationship is a general feature of tight epithelia. Furthermore, the high values of transepithelial resistance imply that the lower limit for the paracellular shunt pathway resistance should be reset upwards.

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References
1.
Walser M . Role of edge damage in sodium permeability of toad bladder and a means of avoiding it. Am J Physiol. 1970; 219(1):252-5. DOI: 10.1152/ajplegacy.1970.219.1.252. View
2.
Helman S, Miller D . In vitro techniques for avoiding edge damage in studies of frog skin. Science. 1971; 173(3992):146-8. DOI: 10.1126/science.173.3992.146. View
3.
HIGGINS Jr J, Cesaro L, Gebler B, Fromter E . Electrical properties of amphibian urinary bladder epithelia. I. Inverse relationship between potential difference and resistance in tightly mounted preparations. Pflugers Arch. 1975; 358(1):41-56. DOI: 10.1007/BF00584568. View
4.
Lewis S, Diamond J . Active sodium transport by mammalian urinary bladder. Nature. 1975; 253(5494):747-8. DOI: 10.1038/253747a0. View
5.
Helman S, Miller D . Edge damage effect on electrical measurements of frog skin. Am J Physiol. 1973; 225(4):972-7. DOI: 10.1152/ajplegacy.1973.225.4.972. View