The Helix-loop-helix Transcription Factor USF (upstream Stimulating Factor) Binds to a Regulatory Sequence of the Human Insulin Gene Enhancer

Overview

Journal Biochem J

Specialty Biochemistry

Date 1993 Oct 1

PMID 8216223

Citations 19

Authors

M L Read

A R Clark

K Docherty

Affiliations

Soon will be listed here.

Abstract

Two important sequence elements, designated insulin enhancer binding site 1 (IEB1) or NIR and IEB2 or FAR, are involved in regulating expression of the rat insulin I gene. These elements bind a helix-loop-helix transcription factor, insulin enhancer factor 1 (IEF1). The IEB1 site is highly conserved among insulin genes but the IEB2 site is not conserved. To investigate the factors binding at the equivalent IEB1 and IEB2 sites in the human insulin gene enhancer, electrophoretic mobility shift assays were performed using a variety of cell extracts and probes specific for the homologous IEB1 and IEB2 sites. The results indicate that a factor with similar tissue distribution and binding characteristics to those of IEF1 binds to the IEB1 site in the human insulin gene, but that a separate factor, identified as the adenovirus major late transcription factor [MLTF, or upstream stimulating factor (USF)] binds to the IEB2 site.

Citing Articles

Protein Kinase CK2 Controls Ca2.1-Dependent Calcium Currents and Insulin Release in Pancreatic β-Cells.

Scheuer R, Philipp S, Becker A, Nalbach L, Ampofo E, Montenarh M Int J Mol Sci. 2020; 21(13).

PMID: 32630015 PMC: 7370021. DOI: 10.3390/ijms21134668.

Functional interplay between the transcription factors USF1 and PDX-1 and protein kinase CK2 in pancreatic β-cells.

Spohrer S, Gross R, Nalbach L, Schwind L, Stumpf H, Menger M Sci Rep. 2017; 7(1):16367.

PMID: 29180680 PMC: 5703852. DOI: 10.1038/s41598-017-16590-0.

Multiple E-boxes in the distal promoter of the rat pyruvate carboxylase gene function as a glucose-responsive element.

Wutthisathapornchai A, Vongpipatana T, Muangsawat S, Boonsaen T, MacDonald M, Jitrapakdee S PLoS One. 2014; 9(7):e102730.

PMID: 25054881 PMC: 4108332. DOI: 10.1371/journal.pone.0102730.

Mutated beta-catenin evades a microRNA-dependent regulatory loop.

Veronese A, Visone R, Consiglio J, Acunzo M, Lupini L, Kim T Proc Natl Acad Sci U S A. 2011; 108(12):4840-5.

PMID: 21383185 PMC: 3064338. DOI: 10.1073/pnas.1101734108.

Chromatin profiling across the human tumour necrosis factor gene locus reveals a complex, cell type-specific landscape with novel regulatory elements.

Taylor J, Wicks K, Vandiedonck C, Knight J Nucleic Acids Res. 2008; 36(15):4845-62.

PMID: 18653526 PMC: 2528168. DOI: 10.1093/nar/gkn444.

References

Sawadogo M, Roeder R . Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region. Cell. 1985; 43(1):165-75. DOI: 10.1016/0092-8674(85)90021-2. View

Reibel L, Besnard C, Lores P, Jami J, Gacon G . An insulinoma nuclear factor binding to GGGCCC motifs in human insulin gene. Nucleic Acids Res. 1993; 21(7):1595-600. PMC: 309368. DOI: 10.1093/nar/21.7.1595. View

Miyamoto N, Moncollin V, Egly J, Chambon P . Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter. EMBO J. 1985; 4(13A):3563-70. PMC: 554698. DOI: 10.1002/j.1460-2075.1985.tb04118.x. View

Chodosh L, Carthew R, Morgan J, Crabtree G, Sharp P . The adenovirus major late transcription factor activates the rat gamma-fibrinogen promoter. Science. 1987; 238(4827):684-8. DOI: 10.1126/science.3672119. View

Karlsson O, Edlund T, MOSS J, Rutter W, Walker M . A mutational analysis of the insulin gene transcription control region: expression in beta cells is dependent on two related sequences within the enhancer. Proc Natl Acad Sci U S A. 1987; 84(24):8819-23. PMC: 299642. DOI: 10.1073/pnas.84.24.8819. View

Carthew R, Chodosh L, Sharp P . The major late transcription factor binds to and activates the mouse metallothionein I promoter. Genes Dev. 1987; 1(9):973-80. DOI: 10.1101/gad.1.9.973. View

Peritz L, Fodor E, Silversides D, Cattini P, Baxter J, Eberhardt N . The human growth hormone gene contains both positive and negative control elements. J Biol Chem. 1988; 263(11):5005-7. View

Ohlsson H, Karlsson O, Edlund T . A beta-cell-specific protein binds to the two major regulatory sequences of the insulin gene enhancer. Proc Natl Acad Sci U S A. 1988; 85(12):4228-31. PMC: 280400. DOI: 10.1073/pnas.85.12.4228. View

Sawadogo M, Van Dyke M, Gregor P, Roeder R . Multiple forms of the human gene-specific transcription factor USF. I. Complete purification and identification of USF from HeLa cell nuclei. J Biol Chem. 1988; 263(24):11985-93. View

10.

Murre C, McCaw P, Baltimore D . A new DNA binding and dimerization motif in immunoglobulin enhancer binding, daughterless, MyoD, and myc proteins. Cell. 1989; 56(5):777-83. DOI: 10.1016/0092-8674(89)90682-x. View

11.

Karlsson O, Walker M, Rutter W, Edlund T . Individual protein-binding domains of the insulin gene enhancer positively activate beta-cell-specific transcription. Mol Cell Biol. 1989; 9(2):823-7. PMC: 362661. DOI: 10.1128/mcb.9.2.823-827.1989. View

12.

Lemaigre F, Courtois S, Lafontaine D, Rousseau G . Evidence that the upstream stimulatory factor and the Sp1 transcription factor bind in vitro to the promoter of the human-growth-hormone gene. Eur J Biochem. 1989; 181(3):555-61. DOI: 10.1111/j.1432-1033.1989.tb14760.x. View

13.

Scotto K, Kaulen H, Roeder R . Positive and negative regulation of the gene for transcription factor IIIA in Xenopus laevis oocytes. Genes Dev. 1989; 3(5):651-62. DOI: 10.1101/gad.3.5.651. View

14.

Schreiber E, Matthias P, Muller M, Schaffner W . Rapid detection of octamer binding proteins with 'mini-extracts', prepared from a small number of cells. Nucleic Acids Res. 1989; 17(15):6419. PMC: 318318. DOI: 10.1093/nar/17.15.6419. View

15.

Vaulont S, Puzenat N, Levrat F, Cognet M, Kahn A, Raymondjean M . Proteins binding to the liver-specific pyruvate kinase gene promoter. A unique combination of known factors. J Mol Biol. 1989; 209(2):205-19. DOI: 10.1016/0022-2836(89)90273-8. View

16.

Boam D, Docherty K . A tissue-specific nuclear factor binds to multiple sites in the human insulin-gene enhancer. Biochem J. 1989; 264(1):233-9. PMC: 1133568. DOI: 10.1042/bj2640233. View

17.

Walker M, Park C, Rosen A, Aronheim A . A cDNA from a mouse pancreatic beta cell encoding a putative transcription factor of the insulin gene. Nucleic Acids Res. 1990; 18(5):1159-66. PMC: 330430. DOI: 10.1093/nar/18.5.1159. View

18.

Boam D, Clark A, Docherty K . Positive and negative regulation of the human insulin gene by multiple trans-acting factors. J Biol Chem. 1990; 265(14):8285-96. View

19.

Kingston R . Transcription control and differentiation: the HLH family, c-myc and C/EBP. Curr Opin Cell Biol. 1989; 1(6):1081-7. DOI: 10.1016/s0955-0674(89)80054-7. View

20.

Shibasaki Y, Sakura H, Takaku F, Kasuga M . Insulin enhancer binding protein has helix-loop-helix structure. Biochem Biophys Res Commun. 1990; 170(1):314-21. DOI: 10.1016/0006-291x(90)91276-x. View