Radioimmunoscintigraphy of Colorectal Cancer with Technetium-99m-labeled Murine Anti-carcinoembryonic Antigen Monoclonal Antibody in Athymic Nude Mice

Overview

Journal Ann Nucl Med

Date 1994 Feb 1

PMID 8204394

Citations 1

Authors

N Watanabe

N Oriuchi

S Sugiyama

M Kuroki

Y Matsuoka

Affiliations

Soon will be listed here.

Abstract

Technetium-99m(Tc-99m) is an ideal radionuclide for clinical use. A murine monoclonal antibody (Mab) designated F33-104 binds to specific parts of carcinoembyronic antigen (CEA). In the present study, intact Mab F33-104 was labeled with Tc-99m, and the immunoreactivity and biodistribution of Tc-99m-labeled F33-104 were studied in athymic nude mice bearing human colorectal cancer xenografts. Mab F33-104, reduced under optimal conditions, was quickly and stably tagged with Tc-99m without loss of immunoreactivity. Higher tumor uptake of Tc-99m-labeled F33-104 was noted in the biodistribution, resulting in a higher localization index and specific-to-non-specific tumor ratio than those of radioiodinated F33-104. These results suggest the potential of Tc-99m-labeled Mab F33-104 for the radioimmunoimaging of colorectal cancer.

Citing Articles

Different biodistribution of 99mTc-labelled chimeric mouse-human monoclonal antibody between athymic mice model and human.

Oriuchi N, Watanabe N, Sugiyama S, Higuchi T, Imai K, Yamanaka H Br J Cancer. 1996; 73(12):1466-72.

PMID: 8664114 PMC: 2074533. DOI: 10.1038/bjc.1996.278.

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