Variations in Radioimmunoscintigraphic Detection of Tumor Showed by Five Monoclonal Antibodies to Carcinoembryonic Antigen

Overview

Journal Ann Nucl Med

Date 1991 Jul 1

PMID 1777353

Citations 1

Authors

K Kohno

K Nakata

Y Kusumoto

N Ishii

T Kohji

Y Matsuoka

S Nishi

S Nagataki

Affiliations

Soon will be listed here.

Abstract

Radioimmunoscintigraphy using mouse monoclonal antibodies to various parts of a carcinoembryonic antigen (CEA) molecule was performed. Four radiolabeled antibodies (F4-82, 28A, F3-30, F33-104) were injected into tumor transplanted nude mice to compare the accumulation of these antibodies in tumors. The four antibodies were accumulated selectively in CEA- producing tumors. The tumor visualization correlated with the tumor/blood radioactivity ratio, whereas the tumor/blood radioactivity ratio did not correlate with the in vitro percent binding to tumor cells or the in vivo percent injected dose in CEA-producing tumors. Among the four antibodies, F33-104 showed the highest tumor/blood radioactivity ratio and the best image quality in any CEA-producing tumor. These results suggest that the antibody which has a high tumor/blood ratio rather than high total tumor uptake may be useful for radioimmunoscintigraphy.

Citing Articles

Radioimmunoscintigraphy of colorectal cancer with technetium-99m-labeled murine anti-carcinoembryonic antigen monoclonal antibody in athymic nude mice.

Watanabe N, Oriuchi N, Sugiyama S, Kuroki M, Matsuoka Y Ann Nucl Med. 1994; 8(1):23-30.

PMID: 8204394 DOI: 10.1007/BF03164983.

References

Kuroki M, Koga Y, Matsuoka Y . Purification and characterization of carcinoembryonic antigen-related antigens in normal adult feces. Cancer Res. 1981; 41(2):713-20. View

Mach J, Forni M, Ritschard J, Buchegger F, Carrel S, Widgren S . Use of limitations of radiolabeled anti-CEA antibodies and their fragments for photoscanning detection of human colorectal carcinomas. Oncodev Biol Med. 1980; 1(1):49-69. View

Kuroki M, Koga Y, Matsuoka Y . Monoclonal antibodies to carcinoembryonic antigen: a systematic analysis of antibody specificities by using related normal antigens and evidence for allotypic determinants on carcinoembryonic antigen. J Immunol. 1984; 133(4):2090-7. View

Pignatelli M, Durbin H, Bodmer W . Carcinoembryonic antigen functions as an accessory adhesion molecule mediating colon epithelial cell-collagen interactions. Proc Natl Acad Sci U S A. 1990; 87(4):1541-5. PMC: 53511. DOI: 10.1073/pnas.87.4.1541. View

Kim E, DeLand F, Casper S, Corgan R, Primus F, Goldenberg D . Radioimmunodetection of colorectal cancer. Cancer. 1980; 45(5 Suppl):1243-7. DOI: 10.1002/1097-0142(19800315)45:5+<1243::aid-cncr2820451334>3.0.co;2-1. View

Sutherland R, Buchegger F, Schreyer M, Vacca A, Mach J . Penetration and binding of radiolabeled anti-carcinoembryonic antigen monoclonal antibodies and their antigen binding fragments in human colon multicellular tumor spheroids. Cancer Res. 1987; 47(6):1627-33. View

Goldenberg D, Kim E, DeLand F, Bennett S, Primus F . Radioimmunodetection of cancer with radioactive antibodies to carcinoembryonic antigen. Cancer Res. 1980; 40(8 Pt 2):2984-92. View

Steward M, Petty R . The use of ammonium sulphate globulin precipitation for determination of affinity of anti-protein antibodies in mouse serum. Immunology. 1972; 22(5):747-56. PMC: 1407860. View

Primus F, Kuhns W, Goldenberg D . Immunological heterogeneity of carcinoembryonic antigen: immunohistochemical detection of carcinoembryonic antigen determinants in colonic tumors with monoclonal antibodies. Cancer Res. 1983; 43(2):693-701. View

10.

Sharkey R, Goldenberg D, Goldenberg H, Lee R, Ballance C, Pawlyk D . Murine monoclonal antibodies against carcinoembryonic antigen: immunological, pharmacokinetic, and targeting properties in humans. Cancer Res. 1990; 50(9):2823-31. View

11.

Salacinski P, Mclean C, Sykes J, Lowry P . Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem. 1981; 117(1):136-46. DOI: 10.1016/0003-2697(81)90703-x. View

12.

Purnell D, COMBS J, Valerio M, Harris C, Trump B . Immunocytochemical evaluation of primary human esophageal carcinomas and their xenografts for keratin, beta-chorionic gonadotropin, placental lactogen, alpha-fetoprotein, carcinoembryonic antigen, and nonspecific cross-reacting antigen. Cancer Res. 1986; 46(11):5730-7. View

13.

Benchimol S, Fuks A, Jothy S, Beauchemin N, Shirota K, Stanners C . Carcinoembryonic antigen, a human tumor marker, functions as an intercellular adhesion molecule. Cell. 1989; 57(2):327-34. DOI: 10.1016/0092-8674(89)90970-7. View

14.

Ishii N, Nakata K, Muro T, Furukawa R, Kono K, Kusumoto Y . Radioimmunodetection of cancer using antibodies to alpha-fetoprotein and carcinoembryonic antigen. Ann N Y Acad Sci. 1983; 417:270-6. DOI: 10.1111/j.1749-6632.1983.tb32870.x. View

15.

Primus F, Newell K, Blue A, Goldenberg D . Immunological heterogeneity of carcinoembryonic antigen: antigenic determinants on carcinoembryonic antigen distinguished by monoclonal antibodies. Cancer Res. 1983; 43(2):686-92. View

16.

Kuroki M, Arakawa F, Higuchi H, Matsunaga A, Okamoto N, Takakura K . Epitope mapping of the carcinoembryonic antigen by monoclonal antibodies and establishment of a new improved radioimmunoassay system. Jpn J Cancer Res. 1987; 78(4):386-96. View

17.

Mach J, Buchegger F, Forni M, Ritschard J, Berche C, Lumbroso J . Use of radiolabelled monoclonal anti-CEA antibodies for the detection of human carcinomas by external photoscanning and tomoscintigraphy. Immunol Today. 2014; 2(12):239-49. DOI: 10.1016/0167-5699(81)90011-6. View

18.

Ahnen D, Kinoshita K, Nakane P, Brown W . Differential expression carcinoembryonic antigen and secretory component during colonic epithelial cell differentiation and in colonic carcinomas. Gastroenterology. 1987; 93(6):1330-8. DOI: 10.1016/0016-5085(87)90263-0. View

19.

Goldenberg D, PRESTON D, Primus F, Hansen H . Photoscan localization of GW-39 tumors in hamsters using radiolabeled anticarcinoembryonic antigen immunoglobulin G. Cancer Res. 1974; 34(1):1-9. View

20.

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View