Isolation of Human T and B Lymphocytes by Rosette Formation with 2-aminoethylisothiquronium Bromide (AET) -treated Sheep Red Blood Cells with Monkey Red Blood Cells

Overview

Journal J Immunol Methods

Publisher Elsevier

Specialties Allergy & Immunology
Pathology

Date 1976 Jan 1

PMID 819587

Citations 32

Authors

M A Pellegrino

S Ferrone

A N Theofilopoulos

Affiliations

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Abstract

Purified human B and T lymphocytes were obtained by rosetting HPL with AET-SRBC or MRBC and separating the non-rosetted from the rosetted cells on Ficoll-Hypaque gradient. 92 +/- 5% of the B cells were fluorescent positive for mBlg and 95 +/- 2% of the purified T cells rosetted with AET-SRBC. 69 +/- 5% of the B cells and 61 +/- 8% of the T cells present in the unifractionated HPL were recovered in the purified fractions.

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