Separation of Immunoreactive Lymphocytes from Human Pluripotent Stem Cells (CFU-GEMM) by Means of Counterflow Centrifugation

Overview

Journal Blut

Specialty Hematology

Date 1984 Mar 1

PMID 6230124

Citations 1

Authors

T de Witte

E Koekman

E Geestman

A Plas

G Blankenborg

J Wessels

C Haanen

Affiliations

Soon will be listed here.

Abstract

Counterflow centrifugation with continuous monitoring of the output for cell number and cell scatter was used to separate low density (d less than 1.070 g/ml) human bone marrow cells in two fractions: one containing the majority of small size lymphocytes and the other the majority of the larger sized committed progenitor cells. The recovery of the pluripotent stem cells (CFU-GEMM) in the large cell fraction was complete. The mitogenic reactivity of this putative stem cell fraction had decreased to 6% and 11%, of the original value as measured with phytohemagglutinin stimulation and one way mixed lymphocytic culture respectively. Counterflow centrifugation offers a physical separation technique, by which the majority of the immunoreactive cells can be separated from the pluripotent hematopoietic stem cells.

Citing Articles

The role of CFU-GEMM in human hemopoiesis.

Messner H Blut. 1986; 53(4):269-77.

PMID: 3530351 DOI: 10.1007/BF00320884.

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